Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. / Stoner, G.D.; Harris, C.C.; Autrup, Herman; Trump, B.F.; Kingsbury, E.W.; Myers, G.A.

In: Laboratory Investigation, Vol. 38, No. 6, 06.1978, p. 658-692.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Stoner, GD, Harris, CC, Autrup, H, Trump, BF, Kingsbury, EW & Myers, GA 1978, 'Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene', Laboratory Investigation, vol. 38, no. 6, pp. 658-692.

APA

Stoner, G. D., Harris, C. C., Autrup, H., Trump, B. F., Kingsbury, E. W., & Myers, G. A. (1978). Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. Laboratory Investigation, 38(6), 658-692.

CBE

Stoner GD, Harris CC, Autrup H, Trump BF, Kingsbury EW, Myers GA. 1978. Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. Laboratory Investigation. 38(6):658-692.

MLA

Stoner, G.D. et al. "Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene". Laboratory Investigation. 1978, 38(6). 658-692.

Vancouver

Stoner GD, Harris CC, Autrup H, Trump BF, Kingsbury EW, Myers GA. Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. Laboratory Investigation. 1978 Jun;38(6):658-692.

Author

Stoner, G.D. ; Harris, C.C. ; Autrup, Herman ; Trump, B.F. ; Kingsbury, E.W. ; Myers, G.A. / Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. In: Laboratory Investigation. 1978 ; Vol. 38, No. 6. pp. 658-692.

Bibtex

@article{ccfe4cd005bc11dbbee902004c4f4f50,
title = "Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene",
abstract = "Human lung explants have been maintained in vitro for a period of 25 days. Autoradiographic studies indicated that the broncholar epithelial cells, type 2 alveolar epithelial cells, and stromal fibroblasts incorporated 3H-thymidine during the culture. After 7 to 10 days, type 2 cells were the predominant alveolar epithelial cell type. Lamellar inclusion bodies were released from the type 2 cells and accumulated in the alveolar spaces. The metabolism of benzo[alpha]pyrene (BP) in human lung explants cultured for up to 7 days was investigated. Human lung explants had measurable aryl hydrocarbon hydroxylase activity and could metabolize BP into forms that were bound to cellular DNA and protein. Peripheral lung had significantly lower aryl hydrocarbon hydroxylase activity than cultured bronchus but both tissues had similar binding levels of BP to DNA. Radioautographic studies indicated that all cell types in the peripheral lung can metabolize BP. The major ethylacetate extractable metabolites of BP formed by peripheral lung were tetrols and trans-7,8-diol. The primary water-soluble metabolite released with arylsulfatase and beta-glucuronidase was 3-hydroxybenzo[alpha]pyrene.",
author = "G.D. Stoner and C.C. Harris and Herman Autrup and B.F. Trump and E.W. Kingsbury and G.A. Myers",
year = "1978",
month = "6",
language = "English",
volume = "38",
pages = "658--692",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene

AU - Stoner, G.D.

AU - Harris, C.C.

AU - Autrup, Herman

AU - Trump, B.F.

AU - Kingsbury, E.W.

AU - Myers, G.A.

PY - 1978/6

Y1 - 1978/6

N2 - Human lung explants have been maintained in vitro for a period of 25 days. Autoradiographic studies indicated that the broncholar epithelial cells, type 2 alveolar epithelial cells, and stromal fibroblasts incorporated 3H-thymidine during the culture. After 7 to 10 days, type 2 cells were the predominant alveolar epithelial cell type. Lamellar inclusion bodies were released from the type 2 cells and accumulated in the alveolar spaces. The metabolism of benzo[alpha]pyrene (BP) in human lung explants cultured for up to 7 days was investigated. Human lung explants had measurable aryl hydrocarbon hydroxylase activity and could metabolize BP into forms that were bound to cellular DNA and protein. Peripheral lung had significantly lower aryl hydrocarbon hydroxylase activity than cultured bronchus but both tissues had similar binding levels of BP to DNA. Radioautographic studies indicated that all cell types in the peripheral lung can metabolize BP. The major ethylacetate extractable metabolites of BP formed by peripheral lung were tetrols and trans-7,8-diol. The primary water-soluble metabolite released with arylsulfatase and beta-glucuronidase was 3-hydroxybenzo[alpha]pyrene.

AB - Human lung explants have been maintained in vitro for a period of 25 days. Autoradiographic studies indicated that the broncholar epithelial cells, type 2 alveolar epithelial cells, and stromal fibroblasts incorporated 3H-thymidine during the culture. After 7 to 10 days, type 2 cells were the predominant alveolar epithelial cell type. Lamellar inclusion bodies were released from the type 2 cells and accumulated in the alveolar spaces. The metabolism of benzo[alpha]pyrene (BP) in human lung explants cultured for up to 7 days was investigated. Human lung explants had measurable aryl hydrocarbon hydroxylase activity and could metabolize BP into forms that were bound to cellular DNA and protein. Peripheral lung had significantly lower aryl hydrocarbon hydroxylase activity than cultured bronchus but both tissues had similar binding levels of BP to DNA. Radioautographic studies indicated that all cell types in the peripheral lung can metabolize BP. The major ethylacetate extractable metabolites of BP formed by peripheral lung were tetrols and trans-7,8-diol. The primary water-soluble metabolite released with arylsulfatase and beta-glucuronidase was 3-hydroxybenzo[alpha]pyrene.

M3 - Journal article

VL - 38

SP - 658

EP - 692

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 6

ER -