Ex Vivo Simultaneous H215O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study

Maibritt Meldgaard Arildsen; Christian Østergaard Mariager; Christoffer Vase Overgaard; Thomas Vorre; Martin Bøjesen; Niels Moeslund; Aage Kristian Olsen Alstrup; Lars Poulsen Tolbod; Mikkel Holm Vendelbo; Steffen Ringgaard; Michael Pedersen; Niels Henrik Buus

doi:10.3390/jimaging10090209

Ex Vivo Simultaneous H₂¹⁵O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study

Maibritt Meldgaard Arildsen, Christian Østergaard Mariager, Christoffer Vase Overgaard, Thomas Vorre, Martin Bøjesen, Niels Moeslund, Aage Kristian Olsen Alstrup, Lars Poulsen Tolbod, Mikkel Holm Vendelbo, Steffen Ringgaard, Michael Pedersen^*, Niels Henrik Buus

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

The aim was to establish combined H₂¹⁵O PET/MRI during ex vivo normothermic machine perfusion (NMP) of isolated porcine kidneys. We examined whether changes in renal arterial blood flow (RABF) are accompanied by changes of a similar magnitude in renal blood perfusion (RBP) as well as the relation between RBP and renal parenchymal oxygenation (RPO). Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a H₂¹⁵O PET sequence for determination of RBP. Results: RBP was measured using H₂¹⁵O PET in all kidneys (flow 1: 0.42–0.76 mL/min/g, flow 2: 0.7–1.6 mL/min/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r² = 0.87). Conclusion: Our study demonstrated the feasibility of combined H₂¹⁵O PET/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.

Original language	English
Article number	209
Journal	Journal of Imaging
Volume	10
Issue	9
ISSN	2313-433X
DOIs	https://doi.org/10.3390/jimaging10090209
Publication status	Published - Sept 2024

Keywords

ex vivo kidney
magnetic resonance imaging
normothermic machine perfusion
positron emission tomography

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10.3390/jimaging10090209

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Arildsen, M. M., Mariager, C. Ø., Overgaard, C. V., Vorre, T., Bøjesen, M., Moeslund, N., Alstrup, A. K. O., Tolbod, L. P., Vendelbo, M. H., Ringgaard, S., Pedersen, M., & Buus, N. H. (2024). Ex Vivo Simultaneous H₂¹⁵O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study. Journal of Imaging, 10(9), Article 209. https://doi.org/10.3390/jimaging10090209

@article{8a284773e82b41009f5f0b64c9fc9e20,

title = "Ex Vivo Simultaneous H215O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study",

abstract = "The aim was to establish combined H215O PET/MRI during ex vivo normothermic machine perfusion (NMP) of isolated porcine kidneys. We examined whether changes in renal arterial blood flow (RABF) are accompanied by changes of a similar magnitude in renal blood perfusion (RBP) as well as the relation between RBP and renal parenchymal oxygenation (RPO). Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a H215O PET sequence for determination of RBP. Results: RBP was measured using H215O PET in all kidneys (flow 1: 0.42–0.76 mL/min/g, flow 2: 0.7–1.6 mL/min/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r2 = 0.87). Conclusion: Our study demonstrated the feasibility of combined H215O PET/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.",

keywords = "ex vivo kidney, magnetic resonance imaging, normothermic machine perfusion, positron emission tomography",

author = "Arildsen, {Maibritt Meldgaard} and Mariager, {Christian {\O}stergaard} and Overgaard, {Christoffer Vase} and Thomas Vorre and Martin B{\o}jesen and Niels Moeslund and Alstrup, {Aage Kristian Olsen} and Tolbod, {Lars Poulsen} and Vendelbo, {Mikkel Holm} and Steffen Ringgaard and Michael Pedersen and Buus, {Niels Henrik}",

note = "Publisher Copyright: {\textcopyright} 2024 by the authors.",

year = "2024",

month = sep,

doi = "10.3390/jimaging10090209",

language = "English",

volume = "10",

journal = "Journal of Imaging",

issn = "2313-433X",

publisher = "Multidisciplinary Digital Publishing Institute",

number = "9",

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Arildsen, MM , Mariager, CØ , Overgaard, CV, Vorre, T, Bøjesen, M, Moeslund, N , Alstrup, AKO , Tolbod, LP , Vendelbo, MH , Ringgaard, S , Pedersen, M & Buus, NH 2024, 'Ex Vivo Simultaneous H₂¹⁵O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study', Journal of Imaging, vol. 10, no. 9, 209. https://doi.org/10.3390/jimaging10090209

Ex Vivo Simultaneous H₂¹⁵O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study. / Arildsen, Maibritt Meldgaard ; Mariager, Christian Østergaard ; Overgaard, Christoffer Vase et al.
In: Journal of Imaging, Vol. 10, No. 9, 209, 09.2024.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Ex Vivo Simultaneous H215O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study

AU - Arildsen, Maibritt Meldgaard

AU - Mariager, Christian Østergaard

AU - Overgaard, Christoffer Vase

AU - Vorre, Thomas

AU - Bøjesen, Martin

AU - Moeslund, Niels

AU - Alstrup, Aage Kristian Olsen

AU - Tolbod, Lars Poulsen

AU - Vendelbo, Mikkel Holm

AU - Ringgaard, Steffen

AU - Pedersen, Michael

AU - Buus, Niels Henrik

PY - 2024/9

Y1 - 2024/9

N2 - The aim was to establish combined H215O PET/MRI during ex vivo normothermic machine perfusion (NMP) of isolated porcine kidneys. We examined whether changes in renal arterial blood flow (RABF) are accompanied by changes of a similar magnitude in renal blood perfusion (RBP) as well as the relation between RBP and renal parenchymal oxygenation (RPO). Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a H215O PET sequence for determination of RBP. Results: RBP was measured using H215O PET in all kidneys (flow 1: 0.42–0.76 mL/min/g, flow 2: 0.7–1.6 mL/min/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r2 = 0.87). Conclusion: Our study demonstrated the feasibility of combined H215O PET/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.

AB - The aim was to establish combined H215O PET/MRI during ex vivo normothermic machine perfusion (NMP) of isolated porcine kidneys. We examined whether changes in renal arterial blood flow (RABF) are accompanied by changes of a similar magnitude in renal blood perfusion (RBP) as well as the relation between RBP and renal parenchymal oxygenation (RPO). Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a H215O PET sequence for determination of RBP. Results: RBP was measured using H215O PET in all kidneys (flow 1: 0.42–0.76 mL/min/g, flow 2: 0.7–1.6 mL/min/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r2 = 0.87). Conclusion: Our study demonstrated the feasibility of combined H215O PET/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.

KW - ex vivo kidney

KW - magnetic resonance imaging

KW - normothermic machine perfusion

KW - positron emission tomography

UR - http://www.scopus.com/inward/record.url?scp=85205317970&partnerID=8YFLogxK

U2 - 10.3390/jimaging10090209

DO - 10.3390/jimaging10090209

M3 - Journal article

C2 - 39330429

AN - SCOPUS:85205317970

SN - 2313-433X

VL - 10

JO - Journal of Imaging

JF - Journal of Imaging

IS - 9

M1 - 209

ER -

Ex Vivo Simultaneous H₂¹⁵O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys—A Feasibility Study

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