Evolution and regulation of the Lotus japonicus LysM receptor gene family

Gitte Vestergaard Lohmann; Yoshikazu Shimoda; Mette Wibroe Nielsen; Frank Grønlund Jørgensen; Christina Grossmann; Niels Sandal; Kirsten Sørensen; Søren Thirup; Lene Heegaard Madsen; Satoshi Tabata; Shusei Sato; Jens Stougaard; Simona Radutoiu

doi:10.1094/MPMI-23-4-0510

Evolution and regulation of the Lotus japonicus LysM receptor gene family

Gitte Vestergaard Lohmann, Yoshikazu Shimoda, Mette Wibroe Nielsen, Frank Grønlund Jørgensen, Christina Grossmann, Niels Sandal, Kirsten Sørensen, Søren Thirup, Lene Heegaard Madsen, Satoshi Tabata, Shusei Sato, Jens Stougaard, Simona Radutoiu

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Abstract

LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.

Original language	English
Journal	Molecular plant-microbe interactions
Volume	23
Issue	4
Pages (from-to)	510-21
Number of pages	11
ISSN	0894-0282
DOIs	https://doi.org/10.1094/MPMI-23-4-0510
Publication status	Published - 2010

Keywords

Base Sequence
Chromosome Mapping
Chromosomes, Plant
Evolution, Molecular
Lotus
Molecular Sequence Data
Multigene Family
Phylogeny
Plant Proteins
Sequence Alignment

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10.1094/MPMI-23-4-0510

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title = "Evolution and regulation of the Lotus japonicus LysM receptor gene family",

abstract = "LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.",

keywords = "Base Sequence, Chromosome Mapping, Chromosomes, Plant, Evolution, Molecular, Lotus, Molecular Sequence Data, Multigene Family, Phylogeny, Plant Proteins, Sequence Alignment",

author = "Lohmann, {Gitte Vestergaard} and Yoshikazu Shimoda and Nielsen, {Mette Wibroe} and J{\o}rgensen, {Frank Gr{\o}nlund} and Christina Grossmann and Niels Sandal and Kirsten S{\o}rensen and S{\o}ren Thirup and Madsen, {Lene Heegaard} and Satoshi Tabata and Shusei Sato and Jens Stougaard and Simona Radutoiu",

year = "2010",

doi = "10.1094/MPMI-23-4-0510",

language = "English",

volume = "23",

pages = "510--21",

journal = "Molecular plant-microbe interactions",

issn = "0894-0282",

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Evolution and regulation of the Lotus japonicus LysM receptor gene family. / Lohmann, Gitte Vestergaard; Shimoda, Yoshikazu; Nielsen, Mette Wibroe et al.
In: Molecular plant-microbe interactions, Vol. 23, No. 4, 2010, p. 510-21.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Evolution and regulation of the Lotus japonicus LysM receptor gene family

AU - Lohmann, Gitte Vestergaard

AU - Shimoda, Yoshikazu

AU - Nielsen, Mette Wibroe

AU - Jørgensen, Frank Grønlund

AU - Grossmann, Christina

AU - Sandal, Niels

AU - Sørensen, Kirsten

AU - Thirup, Søren

AU - Madsen, Lene Heegaard

AU - Tabata, Satoshi

AU - Sato, Shusei

AU - Stougaard, Jens

AU - Radutoiu, Simona

PY - 2010

Y1 - 2010

N2 - LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.

AB - LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.

KW - Base Sequence

KW - Chromosome Mapping

KW - Chromosomes, Plant

KW - Evolution, Molecular

KW - Lotus

KW - Molecular Sequence Data

KW - Multigene Family

KW - Phylogeny

KW - Plant Proteins

KW - Sequence Alignment

U2 - 10.1094/MPMI-23-4-0510

DO - 10.1094/MPMI-23-4-0510

M3 - Journal article

C2 - 20192837

SN - 0894-0282

VL - 23

SP - 510

EP - 521

JO - Molecular plant-microbe interactions

JF - Molecular plant-microbe interactions

IS - 4

ER -

Evolution and regulation of the Lotus japonicus LysM receptor gene family

Abstract

Keywords

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