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Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips

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Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. / De Dios Andres, Paula; Westensee, Isabella N.; Brodszkij, Edit et al.
In: Biomacromolecules, Vol. 22, No. 9, 09.2021, p. 3860-3872.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Harvard

De Dios Andres, P, Westensee, IN, Brodszkij, E, Ramos-Docampo, MA, Gal, N & Städler, B 2021, 'Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips', Biomacromolecules, vol. 22, no. 9, pp. 3860-3872. https://doi.org/10.1021/acs.biomac.1c00686

APA

De Dios Andres, P., Westensee, I. N., Brodszkij, E., Ramos-Docampo, M. A., Gal, N., & Städler, B. (2021). Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. Biomacromolecules, 22(9), 3860-3872. https://doi.org/10.1021/acs.biomac.1c00686

CBE

De Dios Andres P, Westensee IN, Brodszkij E, Ramos-Docampo MA, Gal N, Städler B. 2021. Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. Biomacromolecules. 22(9):3860-3872. https://doi.org/10.1021/acs.biomac.1c00686

MLA

De Dios Andres, Paula et al. "Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips". Biomacromolecules. 2021, 22(9). 3860-3872. https://doi.org/10.1021/acs.biomac.1c00686

Vancouver

De Dios Andres P, Westensee IN, Brodszkij E, Ramos-Docampo MA, Gal N, Städler B. Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. Biomacromolecules. 2021 Sept;22(9):3860-3872. doi: 10.1021/acs.biomac.1c00686

Author

De Dios Andres, Paula ; Westensee, Isabella N. ; Brodszkij, Edit et al. / Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. In: Biomacromolecules. 2021 ; Vol. 22, No. 9. pp. 3860-3872.

Bibtex

@article{939081c0af8c47f5ba79c7e442172628,
title = "Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips",
abstract = "Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.",
author = "{De Dios Andres}, Paula and Westensee, {Isabella N.} and Edit Brodszkij and Ramos-Docampo, {Miguel A.} and Noga Gal and Brigitte St{\"a}dler",
note = "Publisher Copyright: {\textcopyright} 2021 American Chemical Society",
year = "2021",
month = sep,
doi = "10.1021/acs.biomac.1c00686",
language = "English",
volume = "22",
pages = "3860--3872",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "AMER CHEMICAL SOC",
number = "9",

}

RIS

TY - JOUR

T1 - Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips

AU - De Dios Andres, Paula

AU - Westensee, Isabella N.

AU - Brodszkij, Edit

AU - Ramos-Docampo, Miguel A.

AU - Gal, Noga

AU - Städler, Brigitte

N1 - Publisher Copyright: © 2021 American Chemical Society

PY - 2021/9

Y1 - 2021/9

N2 - Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.

AB - Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.

UR - http://www.scopus.com/inward/record.url?scp=85114400619&partnerID=8YFLogxK

U2 - 10.1021/acs.biomac.1c00686

DO - 10.1021/acs.biomac.1c00686

M3 - Journal article

C2 - 34420299

AN - SCOPUS:85114400619

VL - 22

SP - 3860

EP - 3872

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 9

ER -