Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips

Paula De Dios Andres; Isabella N. Westensee; Edit Brodszkij; Miguel A. Ramos-Docampo; Noga Gal; Brigitte Städler

doi:10.1021/acs.biomac.1c00686

Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips

Paula De Dios Andres, Isabella N. Westensee, Edit Brodszkij, Miguel A. Ramos-Docampo, Noga Gal, Brigitte Städler^*

^*Corresponding author for this work

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.

Original language	English
Journal	Biomacromolecules
Volume	22
Issue	9
Pages (from-to)	3860-3872
Number of pages	13
ISSN	1525-7797
DOIs	https://doi.org/10.1021/acs.biomac.1c00686
Publication status	Published - Sept 2021

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@article{939081c0af8c47f5ba79c7e442172628,

title = "Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips",

abstract = "Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.",

author = "{De Dios Andres}, Paula and Westensee, {Isabella N.} and Edit Brodszkij and Ramos-Docampo, {Miguel A.} and Noga Gal and Brigitte St{\"a}dler",

note = "Publisher Copyright: {\textcopyright} 2021 American Chemical Society",

year = "2021",

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doi = "10.1021/acs.biomac.1c00686",

language = "English",

volume = "22",

pages = "3860--3872",

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Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips. / De Dios Andres, Paula; Westensee, Isabella N.; Brodszkij, Edit et al.
In: Biomacromolecules, Vol. 22, No. 9, 09.2021, p. 3860-3872.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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AU - Westensee, Isabella N.

AU - Brodszkij, Edit

AU - Ramos-Docampo, Miguel A.

AU - Gal, Noga

AU - Städler, Brigitte

PY - 2021/9

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N2 - Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.

AB - Cell culture-based intestinal models are important to evaluate nanoformulations intended for oral drug delivery. We report the use of a floating structured paper chip as a scaffold for Caco-2 cells and HT29-MTX-E12 cells that are two established cell types used in intestinal cell models. The formation of cell monolayers for both mono- and cocultures in the paper chip are confirmed and the level of formed cell-cell junctions is evaluated. Further, cocultures show first mucus formation between 6-10 days with the mucus becoming more pronounced after 19 days. Hybrid vesicles (HVs) made from phospholipids and the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) in different ratios are used as a representative soft nanoparticle to assess their mucopenetration ability in paper chip-based cell cultures. The HV assembly is characterized, and it is illustrated that these HVs cross the mucus layer and are found intracellularly within 3 h when the cells are grown in the paper chips. Taken together, the moist three-dimensional cellulose environment of structured paper chips offers an interesting cell culture-based intestinal model that can be further integrated with fluidic systems or online read-out opportunities.

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Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips

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