About Aarhus University
Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
DOI
- https://doi.org/10.1038/s41598-022-13777-y
Final published version
- Mitra Gultom, Federal Food Safety and Veterinary Office, University of Bern ,
- Annika Kratzel, Federal Food Safety and Veterinary Office, University of Bern ,
- Jasmine Portmann, Federal Food Safety and Veterinary Office, University of Bern ,
- Hanspeter Stalder, Federal Food Safety and Veterinary Office, University of Bern ,
- Astrid Chanfon Bätzner, University of Bern ,
- Hans Gantenbein, University of Bern ,
- Corinne Gurtner, University of Bern ,
- Nadine Ebert, Federal Food Safety and Veterinary Office, University of Bern ,
- Hans Henrik Gad
- Rune Hartmann
- Horst Posthaus, University of Bern ,
- Patrik Zanolari, University of Bern ,
- Stephanie Pfaender, Federal Food Safety and Veterinary Office, University of Bern, Ruhr University Bochum ,
- Volker Thiel, Federal Food Safety and Veterinary Office, University of Bern ,
- Ronald Dijkman, Federal Food Safety and Veterinary Office, University of Bern
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.
| Original language | English |
|---|---|
| Article number | 10340 |
| Journal | Scientific Reports |
| Volume | 12 |
| Issue | 1 |
| Number of pages | 9 |
| ISSN | 2045-2322 |
| DOIs | |
| Publication status | Published - Dec 2022 |
See relations at Aarhus University Citationformats
ID: 277882021