Establishing a health-based recommended occupational exposure limit for isoflurane using experimental animal data: a systematic review protocol

Fréderique Struijs, Carlijn R Hooijmans, Marije Buijs, Albert Dahan, Sebastian Hoffmann, Romy Kiffen, Daniele Mandrioli, Julia Menon, Merel Ritskes-Hoitinga, Nel Roeleveld, Anne de Ruijter, Gert Jan Scheffer, Vivi Schlünssen, Paul T J Scheepers*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

Abstract

BACKGROUND: Isoflurane is used as an inhalation anesthetic in medical, paramedical, and veterinary practice. Epidemiological studies suggest an increased risk of miscarriages and malformations at birth related to maternal exposure to isoflurane and other inhalation anesthetics. However, these studies cannot be used to derive an occupational exposure level (OEL), because exposure was not determined quantitatively and other risk factors such as co-exposures to other inhalation anesthetics and other work-related factors may also have contributed to the observed adverse outcomes. The aim of this systematic review project is to assess all available evidence on the effects of isoflurane in studies of controlled exposures in laboratory animals to derive a health-based recommended OEL.

METHODS: A comprehensive search strategy was developed to retrieve all animal studies addressing isoflurane exposure from PubMed, EMBASE, and Web of Science. Title-abstract screening will be performed by machine learning, and full-text screening by one reviewer. Discrepancies will be resolved by discussion. We will include primary research in healthy, sexually mature (non human) vertebrates of single exposure to isoflurane. Studies describing combined exposure and treatments with >  = 1 vol% isoflurane will be excluded. Subsequently, details regarding study identification, study design, animal model, and intervention will be summarized. All relevant exposure characteristics and outcomes will be extracted. The risk of bias will be assessed by two independent reviewers using an adapted version of the SYRCLE's risk of bias tool and an addition of the OHAT tool. For all outcomes for which dose-response curves can be derived, the benchmark dose (BMD) approach will be used to establish a point of departure for deriving a recommended health-based recommended OEL for 8 h (workshift exposure) and for 15 min (short-term exposure).

DISCUSSION: Included studies should be sufficiently sensitive to detect the adverse health outcomes of interest. Uncertainties in the extrapolation from animals to humans will be addressed using assessment factor. These factors are justified in accordance with current practice in chemical risk assessment. A panel of experts will be involved to reach consensus decisions regarding significant steps in this project, such as determination of the critical effects and how to extrapolate from animals to humans.

SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42022308978.

Original languageEnglish
Article number166
JournalSystematic Reviews
Volume12
Issue1
Number of pages8
ISSN2046-4053
DOIs
Publication statusPublished - Dec 2023

Keywords

  • Animals
  • Infant, Newborn
  • Female
  • Humans
  • Isoflurane/adverse effects
  • Anesthetics, Inhalation/toxicity
  • Systematic Reviews as Topic
  • Animals, Laboratory
  • Occupational Exposure/adverse effects
  • Animal studies
  • Inhalation anesthetic
  • Reproductive and developmental toxicity
  • Work place standard
  • Occupational exposure

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