Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves

María B Rabaglino; Jan Bojsen-Møller Secher; Marc-André Sirard; Poul Hyttel; Haja N Kadarmideen

doi:10.1096/fj.202101067R

Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves

María B Rabaglino, Jan Bojsen-Møller Secher, Marc-André Sirard, Poul Hyttel, Haja N Kadarmideen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

In cattle, several calves born after IVP ("in vitro" embryo production) present similar birthweight to those generated after MOET (multiple ovulation and embryo transfer). However, the underlying molecular patterns in organs involved in the developmental process are unknown and could indicate physiological programming. The objectives of this study were: (1) to compare epigenomic and transcriptomic modifications in the hypothalamus, pituitary, gonadal and adrenal organs between 3 months old ovum pick-up-IVP and MOET male calves (n = 4 per group) and (2) to use blood epigenomic data to proxy methylation of the inner organs. Extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA sequencing, respectively. Next, bioinformatic analyses determined differentially methylated cytosines (DMC) and differentially expressed genes (DEG) (FDR

Original language	English
Article number	e21882
Journal	FASEB Journal
Volume	35
Issue	10
Number of pages	19
ISSN	0892-6638
DOIs	https://doi.org/10.1096/fj.202101067R
Publication status	Published - 2021
Externally published	Yes

Keywords

computational biology
machine learning
reproductive techniques
system biology

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10.1096/fj.202101067R

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title = "Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves",

abstract = "In cattle, several calves born after IVP ({"}in vitro{"} embryo production) present similar birthweight to those generated after MOET (multiple ovulation and embryo transfer). However, the underlying molecular patterns in organs involved in the developmental process are unknown and could indicate physiological programming. The objectives of this study were: (1) to compare epigenomic and transcriptomic modifications in the hypothalamus, pituitary, gonadal and adrenal organs between 3 months old ovum pick-up-IVP and MOET male calves (n = 4 per group) and (2) to use blood epigenomic data to proxy methylation of the inner organs. Extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA sequencing, respectively. Next, bioinformatic analyses determined differentially methylated cytosines (DMC) and differentially expressed genes (DEG) (FDR ",

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doi = "10.1096/fj.202101067R",

language = "English",

volume = "35",

journal = "FASEB Journal",

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Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves. / Rabaglino, María B; Bojsen-Møller Secher, Jan; Sirard, Marc-André et al.
In: FASEB Journal, Vol. 35, No. 10, e21882, 2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves

AU - Rabaglino, María B

AU - Bojsen-Møller Secher, Jan

AU - Sirard, Marc-André

AU - Hyttel, Poul

AU - Kadarmideen, Haja N

PY - 2021

Y1 - 2021

N2 - In cattle, several calves born after IVP ("in vitro" embryo production) present similar birthweight to those generated after MOET (multiple ovulation and embryo transfer). However, the underlying molecular patterns in organs involved in the developmental process are unknown and could indicate physiological programming. The objectives of this study were: (1) to compare epigenomic and transcriptomic modifications in the hypothalamus, pituitary, gonadal and adrenal organs between 3 months old ovum pick-up-IVP and MOET male calves (n = 4 per group) and (2) to use blood epigenomic data to proxy methylation of the inner organs. Extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA sequencing, respectively. Next, bioinformatic analyses determined differentially methylated cytosines (DMC) and differentially expressed genes (DEG) (FDR

AB - In cattle, several calves born after IVP ("in vitro" embryo production) present similar birthweight to those generated after MOET (multiple ovulation and embryo transfer). However, the underlying molecular patterns in organs involved in the developmental process are unknown and could indicate physiological programming. The objectives of this study were: (1) to compare epigenomic and transcriptomic modifications in the hypothalamus, pituitary, gonadal and adrenal organs between 3 months old ovum pick-up-IVP and MOET male calves (n = 4 per group) and (2) to use blood epigenomic data to proxy methylation of the inner organs. Extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA sequencing, respectively. Next, bioinformatic analyses determined differentially methylated cytosines (DMC) and differentially expressed genes (DEG) (FDR

KW - computational biology

KW - machine learning

KW - reproductive techniques

KW - system biology

UR - http://www.scopus.com/inward/record.url?scp=85115231274&partnerID=8YFLogxK

U2 - 10.1096/fj.202101067R

DO - 10.1096/fj.202101067R

M3 - Journal article

C2 - 34460963

SN - 0892-6638

VL - 35

JO - FASEB Journal

JF - FASEB Journal

IS - 10

M1 - e21882

ER -

Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves

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Keywords

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