TY - JOUR
T1 - Epigenome editing strategies for plants
T2 - a mini review
AU - Subramanian, Abirami T.
AU - Roy, Priyanka
AU - Aravind, Balamurugan
AU - Kumar, Akash P.
AU - Mohannath, Gireesha
N1 - Publisher Copyright:
© The Author(s) under exclusive licence to Archana Sharma Foundation of Calcutta 2024.
PY - 2024/4
Y1 - 2024/4
N2 - Recent development of genome editing (GE) tools has revolutionized crop improvement due to their ability to perform targeted genetic modifications. Among different generations of GE tools, CRISPR-Cas9 and its variants have become the most preferred tools due to their simplicity compared to the other GE tools. Recent studies have demonstrated employment of modified GE tools to introduce heritable epigenetic changes. In this review, we briefly describe what constitutes epigenome, epigenome editing (EGE), and some of the recently developed protein scaffold platforms such as, SunTag and SSSavi systems, being used for efficient EGE. We then discuss various CRISPR-Cas-based EGE approaches for modulating gene expression and the corresponding phenotype. Mainly, these approaches include targeted DNA methylation/demethylation, histone modification, and base editor-mediated conversion of promoter cytosines to other bases. Of these, the last EGE strategy involves genetic alteration to introduce epigenetic changes. Graphical abstract: (Figure presented.)
AB - Recent development of genome editing (GE) tools has revolutionized crop improvement due to their ability to perform targeted genetic modifications. Among different generations of GE tools, CRISPR-Cas9 and its variants have become the most preferred tools due to their simplicity compared to the other GE tools. Recent studies have demonstrated employment of modified GE tools to introduce heritable epigenetic changes. In this review, we briefly describe what constitutes epigenome, epigenome editing (EGE), and some of the recently developed protein scaffold platforms such as, SunTag and SSSavi systems, being used for efficient EGE. We then discuss various CRISPR-Cas-based EGE approaches for modulating gene expression and the corresponding phenotype. Mainly, these approaches include targeted DNA methylation/demethylation, histone modification, and base editor-mediated conversion of promoter cytosines to other bases. Of these, the last EGE strategy involves genetic alteration to introduce epigenetic changes. Graphical abstract: (Figure presented.)
KW - Base editor
KW - CRISPR-Cas
KW - Epigenome editing
KW - SSSavi
KW - SunTag
UR - https://www.scopus.com/pages/publications/85190154712
U2 - 10.1007/s13237-024-00483-5
DO - 10.1007/s13237-024-00483-5
M3 - Review
AN - SCOPUS:85190154712
SN - 0029-568X
VL - 67
SP - 75
EP - 87
JO - Nucleus: an International Journal of Cytology and Molecular Biology
JF - Nucleus: an International Journal of Cytology and Molecular Biology
IS - 1
ER -