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Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein

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Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein. / Abraham, Saji; Paknikar, Raghavendra; Bhumbra, Samina; Luan, Danny; Venkatareddy, Madhusudan; O'Connor, Christopher; Bitzer, Markus; Fenton, Robert A; Hurd, Toby; Garg, Puneet; Patel, Sanjeevkumar R.

In: American journal of physiology. Renal physiology, Vol. 320, No. 3, 03.2021, p. F404-F417.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Abraham, S, Paknikar, R, Bhumbra, S, Luan, D, Venkatareddy, M, O'Connor, C, Bitzer, M, Fenton, RA, Hurd, T, Garg, P & Patel, SR 2021, 'Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein', American journal of physiology. Renal physiology, vol. 320, no. 3, pp. F404-F417. https://doi.org/10.1152/ajprenal.00371.2020

APA

Abraham, S., Paknikar, R., Bhumbra, S., Luan, D., Venkatareddy, M., O'Connor, C., Bitzer, M., Fenton, R. A., Hurd, T., Garg, P., & Patel, S. R. (2021). Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein. American journal of physiology. Renal physiology, 320(3), F404-F417. https://doi.org/10.1152/ajprenal.00371.2020

CBE

Abraham S, Paknikar R, Bhumbra S, Luan D, Venkatareddy M, O'Connor C, Bitzer M, Fenton RA, Hurd T, Garg P, Patel SR. 2021. Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein. American journal of physiology. Renal physiology. 320(3):F404-F417. https://doi.org/10.1152/ajprenal.00371.2020

MLA

Vancouver

Abraham S, Paknikar R, Bhumbra S, Luan D, Venkatareddy M, O'Connor C et al. Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein. American journal of physiology. Renal physiology. 2021 Mar;320(3):F404-F417. https://doi.org/10.1152/ajprenal.00371.2020

Author

Abraham, Saji ; Paknikar, Raghavendra ; Bhumbra, Samina ; Luan, Danny ; Venkatareddy, Madhusudan ; O'Connor, Christopher ; Bitzer, Markus ; Fenton, Robert A ; Hurd, Toby ; Garg, Puneet ; Patel, Sanjeevkumar R. / Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein. In: American journal of physiology. Renal physiology. 2021 ; Vol. 320, No. 3. pp. F404-F417.

Bibtex

@article{d904373ec1974d15a0d66c29834d19db,
title = "Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein",
abstract = "Renal arginine vasopressin receptor 2 (AVPR2) plays a crucial role in osmoregulation. Engagement of ligand with AVPR2 results in aquaporin 2 movement to the apical membrane and water reabsorption from the urinary filtrate. Despite this essential role, little is known about transcriptional regulation of Avpr2. Here, we identify novel roles for PAX2, a transcription factor crucial for kidney development, and its adaptor protein, Pax transcription interacting protein (PTIP), for epigenetic regulation of Avpr2 and thus body water balance. Chromatin immunoprecipitation (ChIP) from murine inner medulla cells (IMCD-3) identified the minimal DNA-binding region of PAX2 on the Avpr2 promoter. Regulation of Avpr2 by PAX2 was confirmed using a heterologous DNA expression system. PAX2 recruits the adaptor protein PTIP and its associated histone methyltransferase (HMT) complex to Avpr2 promoter, imposing epigenetic marks on this region and throughout the coding sequence that modulate Avpr2 gene transcription. Reduction of PAX2 or PTIP protein levels by siRNA prevented histone lysine methylation and expression of Avpr2. ChIP using mouse or human kidneys determined that PAX2 is highly enriched in the AVPR2 promoter alongside PTIP and HMT proteins, leading to high levels of histone H3 lysine trimethylation within the promoter and throughout the gene. In conclusion, PAX2 provides locus specificity for PTIP, allowing the HMT complex to impart epigenetic changes at the Avpr2 locus and regulate Avpr2 transcription. These finding have major implications for understanding regulation of body water balance.NEW & NOTEWORTHY The transcription factor PAX2 plays an indispensable role in kidney development. In the adult kidney, we identified the first described protein this protein regulates. PAX2 and its interacting partner Pax transcription interacting protein recruit a histone methyltransferase complex to the promoter and epigentically regulate the expression of arginine vasopressin receptor 2, a protein that plays a crucial role in osmoregulation in the distal tubule.",
author = "Saji Abraham and Raghavendra Paknikar and Samina Bhumbra and Danny Luan and Madhusudan Venkatareddy and Christopher O'Connor and Markus Bitzer and Fenton, {Robert A} and Toby Hurd and Puneet Garg and Patel, {Sanjeevkumar R}",
year = "2021",
month = mar,
doi = "10.1152/ajprenal.00371.2020",
language = "English",
volume = "320",
pages = "F404--F417",
journal = "American Journal of Physiology: Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "3",

}

RIS

TY - JOUR

T1 - Epigenetic regulation of arginine vasopressin receptor 2 expression by PAX2 and Pax transcription interacting protein

AU - Abraham, Saji

AU - Paknikar, Raghavendra

AU - Bhumbra, Samina

AU - Luan, Danny

AU - Venkatareddy, Madhusudan

AU - O'Connor, Christopher

AU - Bitzer, Markus

AU - Fenton, Robert A

AU - Hurd, Toby

AU - Garg, Puneet

AU - Patel, Sanjeevkumar R

PY - 2021/3

Y1 - 2021/3

N2 - Renal arginine vasopressin receptor 2 (AVPR2) plays a crucial role in osmoregulation. Engagement of ligand with AVPR2 results in aquaporin 2 movement to the apical membrane and water reabsorption from the urinary filtrate. Despite this essential role, little is known about transcriptional regulation of Avpr2. Here, we identify novel roles for PAX2, a transcription factor crucial for kidney development, and its adaptor protein, Pax transcription interacting protein (PTIP), for epigenetic regulation of Avpr2 and thus body water balance. Chromatin immunoprecipitation (ChIP) from murine inner medulla cells (IMCD-3) identified the minimal DNA-binding region of PAX2 on the Avpr2 promoter. Regulation of Avpr2 by PAX2 was confirmed using a heterologous DNA expression system. PAX2 recruits the adaptor protein PTIP and its associated histone methyltransferase (HMT) complex to Avpr2 promoter, imposing epigenetic marks on this region and throughout the coding sequence that modulate Avpr2 gene transcription. Reduction of PAX2 or PTIP protein levels by siRNA prevented histone lysine methylation and expression of Avpr2. ChIP using mouse or human kidneys determined that PAX2 is highly enriched in the AVPR2 promoter alongside PTIP and HMT proteins, leading to high levels of histone H3 lysine trimethylation within the promoter and throughout the gene. In conclusion, PAX2 provides locus specificity for PTIP, allowing the HMT complex to impart epigenetic changes at the Avpr2 locus and regulate Avpr2 transcription. These finding have major implications for understanding regulation of body water balance.NEW & NOTEWORTHY The transcription factor PAX2 plays an indispensable role in kidney development. In the adult kidney, we identified the first described protein this protein regulates. PAX2 and its interacting partner Pax transcription interacting protein recruit a histone methyltransferase complex to the promoter and epigentically regulate the expression of arginine vasopressin receptor 2, a protein that plays a crucial role in osmoregulation in the distal tubule.

AB - Renal arginine vasopressin receptor 2 (AVPR2) plays a crucial role in osmoregulation. Engagement of ligand with AVPR2 results in aquaporin 2 movement to the apical membrane and water reabsorption from the urinary filtrate. Despite this essential role, little is known about transcriptional regulation of Avpr2. Here, we identify novel roles for PAX2, a transcription factor crucial for kidney development, and its adaptor protein, Pax transcription interacting protein (PTIP), for epigenetic regulation of Avpr2 and thus body water balance. Chromatin immunoprecipitation (ChIP) from murine inner medulla cells (IMCD-3) identified the minimal DNA-binding region of PAX2 on the Avpr2 promoter. Regulation of Avpr2 by PAX2 was confirmed using a heterologous DNA expression system. PAX2 recruits the adaptor protein PTIP and its associated histone methyltransferase (HMT) complex to Avpr2 promoter, imposing epigenetic marks on this region and throughout the coding sequence that modulate Avpr2 gene transcription. Reduction of PAX2 or PTIP protein levels by siRNA prevented histone lysine methylation and expression of Avpr2. ChIP using mouse or human kidneys determined that PAX2 is highly enriched in the AVPR2 promoter alongside PTIP and HMT proteins, leading to high levels of histone H3 lysine trimethylation within the promoter and throughout the gene. In conclusion, PAX2 provides locus specificity for PTIP, allowing the HMT complex to impart epigenetic changes at the Avpr2 locus and regulate Avpr2 transcription. These finding have major implications for understanding regulation of body water balance.NEW & NOTEWORTHY The transcription factor PAX2 plays an indispensable role in kidney development. In the adult kidney, we identified the first described protein this protein regulates. PAX2 and its interacting partner Pax transcription interacting protein recruit a histone methyltransferase complex to the promoter and epigentically regulate the expression of arginine vasopressin receptor 2, a protein that plays a crucial role in osmoregulation in the distal tubule.

U2 - 10.1152/ajprenal.00371.2020

DO - 10.1152/ajprenal.00371.2020

M3 - Journal article

C2 - 33522413

VL - 320

SP - F404-F417

JO - American Journal of Physiology: Renal Physiology

JF - American Journal of Physiology: Renal Physiology

SN - 1931-857X

IS - 3

ER -