Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

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  • Eiichi Murakami
  • ,
  • Jeryl Cheng
  • ,
  • Kira Gysel
  • Zoltan Bozsoki
  • ,
  • Yasuyuki Kawaharada
  • ,
  • Christian Toftegaard Hjuler, Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark.
  • ,
  • Kasper Kildegaard Sørensen, Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark.
  • ,
  • Ke Tao
  • Simon Kelly
  • Francesco Venice, Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
  • ,
  • Andrea Genre, Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
  • ,
  • Mikkel Boas Thygesen, Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark.
  • ,
  • Noor de Jong
  • Maria Vinther
  • Dorthe Bødker Jensen
  • Knud Jørgen Jensen, Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark.
  • ,
  • Michael Blaise
  • ,
  • Lene Heegaard Madsen
  • Kasper Røjkjær Andersen
  • Jens Stougaard
  • Simona Radutoiu

Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.

Original languageEnglish
Article numbere33506
JournaleLife
Volume7
Number of pages21
ISSN2050-084X
DOIs
Publication statusPublished - 29 Jun 2018

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