Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

Eiichi Murakami; Jeryl Cheng; Kira Gysel; Zoltan Bozsoki; Yasuyuki Kawaharada; Christian Toftegaard Hjuler; Kasper Kildegaard Sørensen; Ke Tao; Simon Kelly; Francesco Venice; Andrea Genre; Mikkel Boas Thygesen; Noor de Jong; Maria Vinther; Dorthe Bødker Jensen; Knud Jørgen Jensen; Michael Blaise; Lene Heegaard Madsen; Kasper Røjkjær Andersen; Jens Stougaard; Simona Radutoiu

doi:10.7554/eLife.33506

Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

Eiichi Murakami, Jeryl Cheng, Kira Gysel, Zoltan Bozsoki, Yasuyuki Kawaharada, Christian Toftegaard Hjuler, Kasper Kildegaard Sørensen, Ke Tao, Simon Kelly, Francesco Venice, Andrea Genre, Mikkel Boas Thygesen, Noor de Jong, Maria Vinther, Dorthe Bødker Jensen, Knud Jørgen Jensen, Michael Blaise, Lene Heegaard Madsen, Kasper Røjkjær Andersen, Jens StougaardSimona Radutoiu

Department of Molecular Biology and Genetics - Plant Molecular Biology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.

Original language	English
Article number	e33506
Journal	eLife
Volume	7
Number of pages	21
ISSN	2050-084X
DOIs	https://doi.org/10.7554/eLife.33506
Publication status	Published - 29 Jun 2018

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elife-33506-v1Final published version, 1.25 MBLicence: CC BY

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Murakami, E., Cheng, J., Gysel, K., Bozsoki, Z., Kawaharada, Y., Hjuler, C. T., Sørensen, K. K., Tao, K., Kelly, S., Venice, F., Genre, A., Thygesen, M. B., Jong, N. D., Vinther, M., Jensen, D. B., Jensen, K. J., Blaise, M., Madsen, L. H., Andersen, K. R., ... Radutoiu, S. (2018). Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus. eLife, 7, Article e33506. https://doi.org/10.7554/eLife.33506

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title = "Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus",

abstract = "Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.",

author = "Eiichi Murakami and Jeryl Cheng and Kira Gysel and Zoltan Bozsoki and Yasuyuki Kawaharada and Hjuler, {Christian Toftegaard} and S{\o}rensen, {Kasper Kildegaard} and Ke Tao and Simon Kelly and Francesco Venice and Andrea Genre and Thygesen, {Mikkel Boas} and Jong, {Noor de} and Maria Vinther and Jensen, {Dorthe B{\o}dker} and Jensen, {Knud J{\o}rgen} and Michael Blaise and Madsen, {Lene Heegaard} and Andersen, {Kasper R{\o}jkj{\ae}r} and Jens Stougaard and Simona Radutoiu",

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day = "29",

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Murakami, E, Cheng, J, Gysel, K, Bozsoki, Z, Kawaharada, Y, Hjuler, CT, Sørensen, KK, Tao, K, Kelly, S, Venice, F, Genre, A, Thygesen, MB, Jong, ND , Vinther, M, Jensen, DB, Jensen, KJ, Blaise, M, Madsen, LH, Andersen, KR , Stougaard, J & Radutoiu, S 2018, 'Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus', eLife, vol. 7, e33506. https://doi.org/10.7554/eLife.33506

TY - JOUR

T1 - Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

AU - Murakami, Eiichi

AU - Cheng, Jeryl

AU - Gysel, Kira

AU - Bozsoki, Zoltan

AU - Kawaharada, Yasuyuki

AU - Hjuler, Christian Toftegaard

AU - Sørensen, Kasper Kildegaard

AU - Tao, Ke

AU - Kelly, Simon

AU - Venice, Francesco

AU - Genre, Andrea

AU - Thygesen, Mikkel Boas

AU - Jong, Noor de

AU - Vinther, Maria

AU - Jensen, Dorthe Bødker

AU - Jensen, Knud Jørgen

AU - Blaise, Michael

AU - Madsen, Lene Heegaard

AU - Andersen, Kasper Røjkjær

AU - Stougaard, Jens

AU - Radutoiu, Simona

PY - 2018/6/29

Y1 - 2018/6/29

N2 - Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.

AB - Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.

U2 - 10.7554/eLife.33506

DO - 10.7554/eLife.33506

M3 - Journal article

C2 - 29957177

SN - 2050-084X

VL - 7

JO - eLife

JF - eLife

M1 - e33506

ER -

Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

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