Abstract
Deep eutectic solvents (DESs) have been studied for biocatalysis with hydrolases, oxidoreductases, laccases, lyases, etc., using free enzymes or whole cells. DESs can be used as solvents for biotransformations, enabling the dissolution of substrates with different polarities, opening options for challenging reactions. The addition of water as cosolvent up to 20% (v/v) leads to DES-water mixtures with diminished viscosity but remaining as nonaqueous solvent. For enzyme catalysis, DES can also be used as cosolvents, when water is added in larger amounts (≥20% v/v). In these cases, improvements in enzyme stability and selectivity have been described. Finally, DES can be used as media for multistep catalytic processes, as well as for end-of-pipe tools to separate products and substrates efficiently, after an enzymatic transformation. Overall, DESs represent solid, sustainable, and promising alternatives for biocatalysis.
| Original language | English |
|---|---|
| Title of host publication | Deep Eutectic Solvents : Synthesis, Properties, and Applications |
| Number of pages | 15 |
| Publisher | Wiley |
| Publication date | 1 Jan 2019 |
| Pages | 257-271 |
| ISBN (Print) | 9783527345182 |
| ISBN (Electronic) | 9783527818471 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Keywords
- Biocatalysis
- Deep eutectic solvents
- Downstream processing
- Lipases
- Oxidoreductases
- Whole cells