Enzyme-assisted extraction of rapeseed oil with minimum water addition: a proof-of-concept study

Kristian Thomsen, Norbert Raak*, Sandra Beyer Gregersen, Lars Månsson, Eleonora Miquel Becker

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

1 Citation (Scopus)

Abstract

The increased demand for vegetable oils calls for new strategies for enhancing the yield of mechanical oil extraction, preferably without the use of potentially hazardous solvents such as n-hexane. Enzyme-assisted oil extraction has been studied under aqueous conditions, where the oil recovery was enhanced due to the partial enzymatic breakdown of cell wall compounds. However, this process is often not feasible for industrial applications, where mechanical pressing of dry seeds is implemented. This study provides a proof-of-concept for enzyme-assisted rapeseed oil extraction with minimum water addition (1 mL per 100 g rapeseeds). The application of cellulytic and pectolytic enzymes increased the reducing sugar content as a result of the carbohydrate breakdown. The oil recovery in the mechanical pressing was increased significantly from ~60% to ~65%, and the quality of the oils was not significantly affected by the enzyme treatment.

Original languageEnglish
JournalInternational Journal of Food Science and Technology
Volume59
Issue5
Pages (from-to)3013-3019
Number of pages7
ISSN0950-5423
DOIs
Publication statusPublished - May 2024

Keywords

  • Cellulase
  • enzyme treatment
  • mechanical oil extraction
  • pectinase
  • rapeseed

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