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Enzymatic activity in single cells

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Enzymatic activity in single cells. / Keller, Josephine Geertsen; Stougaard, Magnus; Knudsen, Birgitta R.

Methods in Enzymology. ed. / Nancy L. Allbritton; Michelle L. Kovarik. Elsevier, 2019. p. 43-57 (Methods in Enzymology, Vol. 628).

Research output: Contribution to book/anthology/report/proceedingBook chapterResearchpeer-review

Harvard

Keller, JG, Stougaard, M & Knudsen, BR 2019, Enzymatic activity in single cells. in NL Allbritton & ML Kovarik (eds), Methods in Enzymology. Elsevier, Methods in Enzymology, vol. 628, pp. 43-57. https://doi.org/10.1016/bs.mie.2019.07.003

APA

Keller, J. G., Stougaard, M., & Knudsen, B. R. (2019). Enzymatic activity in single cells. In N. L. Allbritton, & M. L. Kovarik (Eds.), Methods in Enzymology (pp. 43-57). Elsevier. Methods in Enzymology, Vol.. 628 https://doi.org/10.1016/bs.mie.2019.07.003

CBE

Keller JG, Stougaard M, Knudsen BR. 2019. Enzymatic activity in single cells. Allbritton NL, Kovarik ML, editors. In Methods in Enzymology. Elsevier. pp. 43-57. (Methods in Enzymology, Vol. 628). https://doi.org/10.1016/bs.mie.2019.07.003

MLA

Keller, Josephine Geertsen, Magnus Stougaard, and Birgitta R. Knudsen "Enzymatic activity in single cells". and Allbritton, Nancy L. Kovarik, Michelle L. (editors). Methods in Enzymology. Elsevier. (Methods in Enzymology, Vol. 628). 2019, 43-57. https://doi.org/10.1016/bs.mie.2019.07.003

Vancouver

Keller JG, Stougaard M, Knudsen BR. Enzymatic activity in single cells. In Allbritton NL, Kovarik ML, editors, Methods in Enzymology. Elsevier. 2019. p. 43-57. (Methods in Enzymology, Vol. 628). https://doi.org/10.1016/bs.mie.2019.07.003

Author

Keller, Josephine Geertsen ; Stougaard, Magnus ; Knudsen, Birgitta R. / Enzymatic activity in single cells. Methods in Enzymology. editor / Nancy L. Allbritton ; Michelle L. Kovarik. Elsevier, 2019. pp. 43-57 (Methods in Enzymology, Vol. 628).

Bibtex

@inbook{f45643df8e3043479cd6dfa566eb0289,
title = "Enzymatic activity in single cells",
abstract = "With the increasing recognition of the importance in addressing cell-to-cell variations for the understanding of complex biological systems, single cell analyses are becoming increasingly important. Presented in this chapter is a highly sensitive approach capable of measuring human topoisomerase 1 (TOP1) activity in single CD133 positive DLD-1 cells. The method termed On-Slide “Rolling circle Enhanced Enzyme Activity Detection (REEAD)” relies on the specific capture and lysis of CD133 positive cells on glass slides dual functionalized with anti-CD133 antibodies and a specific DNA primer. The On-Slide REEAD was demonstrated to be directly quantitative. Furthermore, the method allowed for the highly sensitive detection of TOP1 activity in single CD133 positive DLD-1 cells. The described protocol is expected to open for new possibilities in the single cell research, particularly for the investigations of chemoresistance of the cancer stem cells.",
keywords = "Cancer, Cancer stem cells, Enzyme activity, Human topoisomerase 1, Microscopic slides, Rolling circle amplification, Single cell detection",
author = "Keller, {Josephine Geertsen} and Magnus Stougaard and Knudsen, {Birgitta R.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/bs.mie.2019.07.003",
language = "English",
isbn = "9780128170908",
series = "Methods in Enzymology",
publisher = "Elsevier",
pages = "43--57",
editor = "Allbritton, {Nancy L.} and Kovarik, {Michelle L.}",
booktitle = "Methods in Enzymology",

}

RIS

TY - CHAP

T1 - Enzymatic activity in single cells

AU - Keller, Josephine Geertsen

AU - Stougaard, Magnus

AU - Knudsen, Birgitta R.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - With the increasing recognition of the importance in addressing cell-to-cell variations for the understanding of complex biological systems, single cell analyses are becoming increasingly important. Presented in this chapter is a highly sensitive approach capable of measuring human topoisomerase 1 (TOP1) activity in single CD133 positive DLD-1 cells. The method termed On-Slide “Rolling circle Enhanced Enzyme Activity Detection (REEAD)” relies on the specific capture and lysis of CD133 positive cells on glass slides dual functionalized with anti-CD133 antibodies and a specific DNA primer. The On-Slide REEAD was demonstrated to be directly quantitative. Furthermore, the method allowed for the highly sensitive detection of TOP1 activity in single CD133 positive DLD-1 cells. The described protocol is expected to open for new possibilities in the single cell research, particularly for the investigations of chemoresistance of the cancer stem cells.

AB - With the increasing recognition of the importance in addressing cell-to-cell variations for the understanding of complex biological systems, single cell analyses are becoming increasingly important. Presented in this chapter is a highly sensitive approach capable of measuring human topoisomerase 1 (TOP1) activity in single CD133 positive DLD-1 cells. The method termed On-Slide “Rolling circle Enhanced Enzyme Activity Detection (REEAD)” relies on the specific capture and lysis of CD133 positive cells on glass slides dual functionalized with anti-CD133 antibodies and a specific DNA primer. The On-Slide REEAD was demonstrated to be directly quantitative. Furthermore, the method allowed for the highly sensitive detection of TOP1 activity in single CD133 positive DLD-1 cells. The described protocol is expected to open for new possibilities in the single cell research, particularly for the investigations of chemoresistance of the cancer stem cells.

KW - Cancer

KW - Cancer stem cells

KW - Enzyme activity

KW - Human topoisomerase 1

KW - Microscopic slides

KW - Rolling circle amplification

KW - Single cell detection

UR - http://www.scopus.com/inward/record.url?scp=85071296425&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2019.07.003

DO - 10.1016/bs.mie.2019.07.003

M3 - Book chapter

C2 - 31668235

AN - SCOPUS:85071296425

SN - 9780128170908

T3 - Methods in Enzymology

SP - 43

EP - 57

BT - Methods in Enzymology

A2 - Allbritton, Nancy L.

A2 - Kovarik, Michelle L.

PB - Elsevier

ER -