Engineered nanostructured β-sheet peptides protect membrane proteins

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Houchao Tao, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA., Denmark
  • Sung Chang Lee, Denmark
  • Arne Möller, The Scripps Research Institute, Denmark
  • Rituparna Sinha Roy, Denmark
  • Fai Yiu Siu, Denmark
  • Jörg Zimmermann, Denmark
  • Raymond C Stevens, Denmark
  • Clinton S Potter, Denmark
  • Bridget Carragher
  • ,
  • Qinghai Zhang, Denmark

We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.

Original languageEnglish
JournalNature Methods
Volume10
Issue8
Pages (from-to)759-61
Number of pages3
ISSN1548-7091
DOIs
Publication statusPublished - Aug 2013
Externally publishedYes

    Research areas

  • ATP-Binding Cassette Transporters, Bacterial Proteins, Circular Dichroism, Membrane Proteins, Microscopy, Electron, Transmission, Nanostructures, Peptides, Protein Structure, Secondary, Spectroscopy, Fourier Transform Infrared

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