Endothelial dysfunction in small arteries and early signs of atherosclerosis in ApoE knockout rats

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Endothelial dysfunction in small arteries and early signs of atherosclerosis in ApoE knockout rats. / Berenji Ardestani, Simin; Eftedal, Ingrid; Pedersen, Michael; Jeppesen, Per Bendix; Nørregaard, Rikke; Matchkov, Vladimir.

In: Scientific Reports, Vol. 10, 15296 , 2020.

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@article{cc9bc0c2f7f04e06bcf747cd2cde4065,
title = "Endothelial dysfunction in small arteries and early signs of atherosclerosis in ApoE knockout rats",
abstract = "Endothelial dysfunction is recognized as a major contributor to atherosclerosis and has been suggested to be evident far before plaque formation. Endothelial dysfunction in small resistance arteries has been suggested to initiate long before changes in conduit arteries. In this study, we address early changes in endothelial function of atherosclerosis prone rats. Male ApoE knockout (KO) rats (11- to 13-weeks-old) were subjected to either a Western or standard diet. The diet intervention continued for a period of 20–24 weeks. Endothelial function of pulmonary and mesenteric arteries was examined in vitro using an isometric myograph. We found that Western diet decreased the contribution of cyclooxygenase (COX) to control the vascular tone of both pulmonary and mesenteric arteries. These changes were associated with early stage atherosclerosis and elevated level of plasma total cholesterol, LDL and triglyceride in ApoE KO rats. Chondroid-transformed smooth muscle cells, calcifications, macrophages accumulation and foam cells were also observed in the aortic arch from ApoE KO rats fed Western diet. The ApoE KO rats are a new model to study endothelial dysfunction during the earlier stages of atherosclerosis and could help us improve preclinical drug development.",
author = "{Berenji Ardestani}, Simin and Ingrid Eftedal and Michael Pedersen and Jeppesen, {Per Bendix} and Rikke N{\o}rregaard and Vladimir Matchkov",
year = "2020",
doi = "10.1038/s41598-020-72338-3",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Endothelial dysfunction in small arteries and early signs of atherosclerosis in ApoE knockout rats

AU - Berenji Ardestani, Simin

AU - Eftedal, Ingrid

AU - Pedersen, Michael

AU - Jeppesen, Per Bendix

AU - Nørregaard, Rikke

AU - Matchkov, Vladimir

PY - 2020

Y1 - 2020

N2 - Endothelial dysfunction is recognized as a major contributor to atherosclerosis and has been suggested to be evident far before plaque formation. Endothelial dysfunction in small resistance arteries has been suggested to initiate long before changes in conduit arteries. In this study, we address early changes in endothelial function of atherosclerosis prone rats. Male ApoE knockout (KO) rats (11- to 13-weeks-old) were subjected to either a Western or standard diet. The diet intervention continued for a period of 20–24 weeks. Endothelial function of pulmonary and mesenteric arteries was examined in vitro using an isometric myograph. We found that Western diet decreased the contribution of cyclooxygenase (COX) to control the vascular tone of both pulmonary and mesenteric arteries. These changes were associated with early stage atherosclerosis and elevated level of plasma total cholesterol, LDL and triglyceride in ApoE KO rats. Chondroid-transformed smooth muscle cells, calcifications, macrophages accumulation and foam cells were also observed in the aortic arch from ApoE KO rats fed Western diet. The ApoE KO rats are a new model to study endothelial dysfunction during the earlier stages of atherosclerosis and could help us improve preclinical drug development.

AB - Endothelial dysfunction is recognized as a major contributor to atherosclerosis and has been suggested to be evident far before plaque formation. Endothelial dysfunction in small resistance arteries has been suggested to initiate long before changes in conduit arteries. In this study, we address early changes in endothelial function of atherosclerosis prone rats. Male ApoE knockout (KO) rats (11- to 13-weeks-old) were subjected to either a Western or standard diet. The diet intervention continued for a period of 20–24 weeks. Endothelial function of pulmonary and mesenteric arteries was examined in vitro using an isometric myograph. We found that Western diet decreased the contribution of cyclooxygenase (COX) to control the vascular tone of both pulmonary and mesenteric arteries. These changes were associated with early stage atherosclerosis and elevated level of plasma total cholesterol, LDL and triglyceride in ApoE KO rats. Chondroid-transformed smooth muscle cells, calcifications, macrophages accumulation and foam cells were also observed in the aortic arch from ApoE KO rats fed Western diet. The ApoE KO rats are a new model to study endothelial dysfunction during the earlier stages of atherosclerosis and could help us improve preclinical drug development.

U2 - 10.1038/s41598-020-72338-3

DO - 10.1038/s41598-020-72338-3

M3 - Journal article

C2 - 32943715

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 15296

ER -