Lotus japonicus ARPC1 is required for Rhizobial Infection

Md Shakhawat Hossain; Jinqiu Liao; Euan K James; Shusei Sato; Satoshi Tabata; Anna Jurkiewicz; Lene Heegaard Madsen; Jens Stougaard; Loretta Ross; Krzysztof Szczyglowski

doi:10.1104/pp.112.202572

Lotus japonicus ARPC1 is required for Rhizobial Infection

Md Shakhawat Hossain, Jinqiu Liao, Euan K James, Shusei Sato, Satoshi Tabata, Anna Jurkiewicz, Lene Heegaard Madsen, Jens Stougaard, Loretta Ross, Krzysztof Szczyglowski

Department of Molecular Biology and Genetics

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

62 Citations (Scopus)

Abstract

Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.

Original language	English
Journal	Plant Physiology
Volume	160
Issue	2
Pages (from-to)	917-928
Number of pages	12
ISSN	0032-0889
DOIs	https://doi.org/10.1104/pp.112.202572
Publication status	Published - 3 Aug 2012

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@article{f8f824108e26426aae79f55a14b41d2a,

title = "Lotus japonicus ARPC1 is required for Rhizobial Infection",

abstract = "Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.",

author = "Hossain, {Md Shakhawat} and Jinqiu Liao and James, {Euan K} and Shusei Sato and Satoshi Tabata and Anna Jurkiewicz and Madsen, {Lene Heegaard} and Jens Stougaard and Loretta Ross and Krzysztof Szczyglowski",

year = "2012",

month = aug,

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doi = "10.1104/pp.112.202572",

language = "English",

volume = "160",

pages = "917--928",

journal = "Plant Physiology",

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publisher = "American Society of Plant Biologists",

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T1 - Lotus japonicus ARPC1 is required for Rhizobial Infection

AU - Hossain, Md Shakhawat

AU - Liao, Jinqiu

AU - James, Euan K

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Jurkiewicz, Anna

AU - Madsen, Lene Heegaard

AU - Stougaard, Jens

AU - Ross, Loretta

AU - Szczyglowski, Krzysztof

PY - 2012/8/3

Y1 - 2012/8/3

N2 - Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.

AB - Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.

U2 - 10.1104/pp.112.202572

DO - 10.1104/pp.112.202572

M3 - Journal article

C2 - 22864583

SN - 0032-0889

VL - 160

SP - 917

EP - 928

JO - Plant Physiology

JF - Plant Physiology

IS - 2

ER -

Lotus japonicus ARPC1 is required for Rhizobial Infection

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