Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy. / Lincke, Kasper; Langeland, Jeppe; Madsen, Andreas Ostergaard; Kiefer, Hjalte V.; Skov, Louise; Gruber, Elisabeth; Mikkelsen, Kurt V.; Andersen, Lars H.; Nielsen, Mogens Brondsted.

In: Physical Chemistry Chemical Physics, Vol. 20, No. 45, 07.12.2018, p. 28678-28684.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Lincke, K, Langeland, J, Madsen, AO, Kiefer, HV, Skov, L, Gruber, E, Mikkelsen, KV, Andersen, LH & Nielsen, MB 2018, 'Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy', Physical Chemistry Chemical Physics, vol. 20, no. 45, pp. 28678-28684. https://doi.org/10.1039/c8cp05368e

APA

Lincke, K., Langeland, J., Madsen, A. O., Kiefer, H. V., Skov, L., Gruber, E., ... Nielsen, M. B. (2018). Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy. Physical Chemistry Chemical Physics, 20(45), 28678-28684. https://doi.org/10.1039/c8cp05368e

CBE

Lincke K, Langeland J, Madsen AO, Kiefer HV, Skov L, Gruber E, Mikkelsen KV, Andersen LH, Nielsen MB. 2018. Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy. Physical Chemistry Chemical Physics. 20(45):28678-28684. https://doi.org/10.1039/c8cp05368e

MLA

Vancouver

Lincke K, Langeland J, Madsen AO, Kiefer HV, Skov L, Gruber E et al. Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy. Physical Chemistry Chemical Physics. 2018 Dec 7;20(45):28678-28684. https://doi.org/10.1039/c8cp05368e

Author

Lincke, Kasper ; Langeland, Jeppe ; Madsen, Andreas Ostergaard ; Kiefer, Hjalte V. ; Skov, Louise ; Gruber, Elisabeth ; Mikkelsen, Kurt V. ; Andersen, Lars H. ; Nielsen, Mogens Brondsted. / Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy. In: Physical Chemistry Chemical Physics. 2018 ; Vol. 20, No. 45. pp. 28678-28684.

Bibtex

@article{e2c1ad70de2d4e3dbc68685ce5f9dc61,
title = "Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy",
abstract = "A model system of the flavin chromophore was synthesized and investigated for its intrinsic optical properties by gas phase action spectroscopy using an ion storage ring. An ammonium group was anchored to this flavin chromophore to allow its transfer to the gas phase by electrospray ionization and for studying the influence of hydrogen bonding and a nearby positive charge. According to calculations one of the hydrogen atoms of the ammonium group favorably forms an intramolecular ionic hydrogen bond to one of the oxygen atoms of the flavin chromophore, and this interaction was found to cause a blueshift of the S-0 S-1 transition and a redshift of the S-0 S-2 transition. For comparison, the S-0 S-1 transition shows little solvent dependence (only in regard to the degree of fine structure). In addition, the influence of protonation of the flavin chromophore was elucidated by experimental and theoretical studies of a simple flavin system. While the position of the S-0 S-1 absorption was at identical positions in the gas phase for the intramolecularly hydrogen-bonded and protonated flavin systems, the S-0 S-2 absorption was further redshifted for the protonated species. This redshift resulting from protonation was also observed in solution.",
keywords = "ELECTROSTATIC STORAGE-RING, BLUE-LIGHT RECEPTOR, ABSORPTION-SPECTRUM, FAD BLUF, RIBOFLAVIN, SLR1694, MODEL, PHOTOPHYSICS, DYNAMICS, SENSOR",
author = "Kasper Lincke and Jeppe Langeland and Madsen, {Andreas Ostergaard} and Kiefer, {Hjalte V.} and Louise Skov and Elisabeth Gruber and Mikkelsen, {Kurt V.} and Andersen, {Lars H.} and Nielsen, {Mogens Brondsted}",
year = "2018",
month = "12",
day = "7",
doi = "10.1039/c8cp05368e",
language = "English",
volume = "20",
pages = "28678--28684",
journal = "Physical Chemistry Chemical Physics",
issn = "1463-9076",
publisher = "ROYAL SOC CHEMISTRY",
number = "45",

}

RIS

TY - JOUR

T1 - Elucidation of the intrinsic optical properties of hydrogen-bonded and protonated flavin chromophores by photodissociation action spectroscopy

AU - Lincke, Kasper

AU - Langeland, Jeppe

AU - Madsen, Andreas Ostergaard

AU - Kiefer, Hjalte V.

AU - Skov, Louise

AU - Gruber, Elisabeth

AU - Mikkelsen, Kurt V.

AU - Andersen, Lars H.

AU - Nielsen, Mogens Brondsted

PY - 2018/12/7

Y1 - 2018/12/7

N2 - A model system of the flavin chromophore was synthesized and investigated for its intrinsic optical properties by gas phase action spectroscopy using an ion storage ring. An ammonium group was anchored to this flavin chromophore to allow its transfer to the gas phase by electrospray ionization and for studying the influence of hydrogen bonding and a nearby positive charge. According to calculations one of the hydrogen atoms of the ammonium group favorably forms an intramolecular ionic hydrogen bond to one of the oxygen atoms of the flavin chromophore, and this interaction was found to cause a blueshift of the S-0 S-1 transition and a redshift of the S-0 S-2 transition. For comparison, the S-0 S-1 transition shows little solvent dependence (only in regard to the degree of fine structure). In addition, the influence of protonation of the flavin chromophore was elucidated by experimental and theoretical studies of a simple flavin system. While the position of the S-0 S-1 absorption was at identical positions in the gas phase for the intramolecularly hydrogen-bonded and protonated flavin systems, the S-0 S-2 absorption was further redshifted for the protonated species. This redshift resulting from protonation was also observed in solution.

AB - A model system of the flavin chromophore was synthesized and investigated for its intrinsic optical properties by gas phase action spectroscopy using an ion storage ring. An ammonium group was anchored to this flavin chromophore to allow its transfer to the gas phase by electrospray ionization and for studying the influence of hydrogen bonding and a nearby positive charge. According to calculations one of the hydrogen atoms of the ammonium group favorably forms an intramolecular ionic hydrogen bond to one of the oxygen atoms of the flavin chromophore, and this interaction was found to cause a blueshift of the S-0 S-1 transition and a redshift of the S-0 S-2 transition. For comparison, the S-0 S-1 transition shows little solvent dependence (only in regard to the degree of fine structure). In addition, the influence of protonation of the flavin chromophore was elucidated by experimental and theoretical studies of a simple flavin system. While the position of the S-0 S-1 absorption was at identical positions in the gas phase for the intramolecularly hydrogen-bonded and protonated flavin systems, the S-0 S-2 absorption was further redshifted for the protonated species. This redshift resulting from protonation was also observed in solution.

KW - ELECTROSTATIC STORAGE-RING

KW - BLUE-LIGHT RECEPTOR

KW - ABSORPTION-SPECTRUM

KW - FAD BLUF

KW - RIBOFLAVIN

KW - SLR1694

KW - MODEL

KW - PHOTOPHYSICS

KW - DYNAMICS

KW - SENSOR

U2 - 10.1039/c8cp05368e

DO - 10.1039/c8cp05368e

M3 - Journal article

VL - 20

SP - 28678

EP - 28684

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 45

ER -