Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells

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Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells. / Laursen, Emma B; Fredsøe, Jacob; Schmidt, Linnéa; Strand, Siri; Kristensen, Helle; Ildor Rasmussen, Anne K; Daugaard, Tina F; Mouritzen, Peter; Høyer, Søren; Kristensen, Gitte; Stroomberg, Hein V; Brasso, Klaus; Røder, Martin A; Borre, Michael; Sørensen, Karina D.

In: The American Journal of Pathology, Vol. 189, No. 12, 12.2019, p. 2377-2388.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Laursen, EB, Fredsøe, J, Schmidt, L, Strand, S, Kristensen, H, Ildor Rasmussen, AK, Daugaard, TF, Mouritzen, P, Høyer, S, Kristensen, G, Stroomberg, HV, Brasso, K, Røder, MA, Borre, M & Sørensen, KD 2019, 'Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells', The American Journal of Pathology, vol. 189, no. 12, pp. 2377-2388. https://doi.org/10.1016/j.ajpath.2019.08.007

APA

Laursen, E. B., Fredsøe, J., Schmidt, L., Strand, S., Kristensen, H., Ildor Rasmussen, A. K., Daugaard, T. F., Mouritzen, P., Høyer, S., Kristensen, G., Stroomberg, H. V., Brasso, K., Røder, M. A., Borre, M., & Sørensen, K. D. (2019). Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells. The American Journal of Pathology, 189(12), 2377-2388. https://doi.org/10.1016/j.ajpath.2019.08.007

CBE

Laursen EB, Fredsøe J, Schmidt L, Strand S, Kristensen H, Ildor Rasmussen AK, Daugaard TF, Mouritzen P, Høyer S, Kristensen G, Stroomberg HV, Brasso K, Røder MA, Borre M, Sørensen KD. 2019. Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells. The American Journal of Pathology. 189(12):2377-2388. https://doi.org/10.1016/j.ajpath.2019.08.007

MLA

Vancouver

Author

Laursen, Emma B ; Fredsøe, Jacob ; Schmidt, Linnéa ; Strand, Siri ; Kristensen, Helle ; Ildor Rasmussen, Anne K ; Daugaard, Tina F ; Mouritzen, Peter ; Høyer, Søren ; Kristensen, Gitte ; Stroomberg, Hein V ; Brasso, Klaus ; Røder, Martin A ; Borre, Michael ; Sørensen, Karina D. / Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells. In: The American Journal of Pathology. 2019 ; Vol. 189, No. 12. pp. 2377-2388.

Bibtex

@article{121b2103bc3640cabd8de95ade31b198,
title = "Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells",
abstract = "miR-615-3p has previously been described as up-regulated in prostate cancer (PC) tissue samples compared with nonmalignant controls; however, its prognostic potential and functional role in PC remain largely unknown. In this study, we investigated the clinical and biological relevance of miR-615-3p in PC. The expression of miR-615-3p was measured in PC tissue specimens from 239 men who underwent radical prostatectomy (RP), and it was investigated if miR-615-3p could predict postoperative biochemical recurrence (BCR). These findings were subsequently validated in three independent RP cohorts (n = 222, n = 273, and n = 387) and functional overexpression studies conducted in PC cells (PC3M). High miR-615-3p expression was significantly associated with BCR in four independent PC patient cohorts (P < 0.05, log-rank test). In addition, high miR-615-3p expression was a significant predictor of PC-specific survival in univariate (hazard ratio, 3.75; P < 0.001) and multivariate (hazard ratio, 2.66; P = 0.008) analysis after adjustment for the CAPRA-S nomogram in a merged RP cohort (N = 734). Moreover, overexpression of miR-615-3p in PC cells (PC3M) significantly increased cell viability, proliferation, apoptosis, and migration. Together, our results suggest that miR-615-3p is a significant predictor of postoperative BCR and PC-specific survival and has oncogenic functions in PC cells.",
author = "Laursen, {Emma B} and Jacob Freds{\o}e and Linn{\'e}a Schmidt and Siri Strand and Helle Kristensen and {Ildor Rasmussen}, {Anne K} and Daugaard, {Tina F} and Peter Mouritzen and S{\o}ren H{\o}yer and Gitte Kristensen and Stroomberg, {Hein V} and Klaus Brasso and R{\o}der, {Martin A} and Michael Borre and S{\o}rensen, {Karina D}",
note = "Copyright {\textcopyright} 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.",
year = "2019",
month = dec,
doi = "10.1016/j.ajpath.2019.08.007",
language = "English",
volume = "189",
pages = "2377--2388",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "12",

}

RIS

TY - JOUR

T1 - Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells

AU - Laursen, Emma B

AU - Fredsøe, Jacob

AU - Schmidt, Linnéa

AU - Strand, Siri

AU - Kristensen, Helle

AU - Ildor Rasmussen, Anne K

AU - Daugaard, Tina F

AU - Mouritzen, Peter

AU - Høyer, Søren

AU - Kristensen, Gitte

AU - Stroomberg, Hein V

AU - Brasso, Klaus

AU - Røder, Martin A

AU - Borre, Michael

AU - Sørensen, Karina D

N1 - Copyright © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

PY - 2019/12

Y1 - 2019/12

N2 - miR-615-3p has previously been described as up-regulated in prostate cancer (PC) tissue samples compared with nonmalignant controls; however, its prognostic potential and functional role in PC remain largely unknown. In this study, we investigated the clinical and biological relevance of miR-615-3p in PC. The expression of miR-615-3p was measured in PC tissue specimens from 239 men who underwent radical prostatectomy (RP), and it was investigated if miR-615-3p could predict postoperative biochemical recurrence (BCR). These findings were subsequently validated in three independent RP cohorts (n = 222, n = 273, and n = 387) and functional overexpression studies conducted in PC cells (PC3M). High miR-615-3p expression was significantly associated with BCR in four independent PC patient cohorts (P < 0.05, log-rank test). In addition, high miR-615-3p expression was a significant predictor of PC-specific survival in univariate (hazard ratio, 3.75; P < 0.001) and multivariate (hazard ratio, 2.66; P = 0.008) analysis after adjustment for the CAPRA-S nomogram in a merged RP cohort (N = 734). Moreover, overexpression of miR-615-3p in PC cells (PC3M) significantly increased cell viability, proliferation, apoptosis, and migration. Together, our results suggest that miR-615-3p is a significant predictor of postoperative BCR and PC-specific survival and has oncogenic functions in PC cells.

AB - miR-615-3p has previously been described as up-regulated in prostate cancer (PC) tissue samples compared with nonmalignant controls; however, its prognostic potential and functional role in PC remain largely unknown. In this study, we investigated the clinical and biological relevance of miR-615-3p in PC. The expression of miR-615-3p was measured in PC tissue specimens from 239 men who underwent radical prostatectomy (RP), and it was investigated if miR-615-3p could predict postoperative biochemical recurrence (BCR). These findings were subsequently validated in three independent RP cohorts (n = 222, n = 273, and n = 387) and functional overexpression studies conducted in PC cells (PC3M). High miR-615-3p expression was significantly associated with BCR in four independent PC patient cohorts (P < 0.05, log-rank test). In addition, high miR-615-3p expression was a significant predictor of PC-specific survival in univariate (hazard ratio, 3.75; P < 0.001) and multivariate (hazard ratio, 2.66; P = 0.008) analysis after adjustment for the CAPRA-S nomogram in a merged RP cohort (N = 734). Moreover, overexpression of miR-615-3p in PC cells (PC3M) significantly increased cell viability, proliferation, apoptosis, and migration. Together, our results suggest that miR-615-3p is a significant predictor of postoperative BCR and PC-specific survival and has oncogenic functions in PC cells.

U2 - 10.1016/j.ajpath.2019.08.007

DO - 10.1016/j.ajpath.2019.08.007

M3 - Journal article

C2 - 31539518

VL - 189

SP - 2377

EP - 2388

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 12

ER -