Electrospun PVA-PCL-HAB scaffold for craniofacial bone regeneration

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  • Rahul Prabha, Endocrine Research laboratory (KMEB), Department of Endocrinology, University Hospital of Odense
  • ,
  • David Christian Evar Kraft
  • Birte Melsen
  • ,
  • H. Varma, Sree Chitra Tirunal Institute for Medical Sciences and Technology(SCTIMST), Trivandrum, India
  • P. D. Nair, Sree Chitra Tirunal Institute for Medical Sciences and Technology(SCTIMST), Trivandrum, India
  • Jørgen Kjems
  • Moustapha Kassem, Syddansk Universitet
Bone tissue engineering for craniofacial region is considered challenging owing to its physiologic and anatomical complexities. A porous bioactive scaffold promoting osteogenesis and angio- genesis is required for clinical applications. We have developed an electrospun polyvinyl alcohol (PVA) poly-caprolactone (PCL)- triphasic bioceramic(HAB) scaffold to biomimic native tissue and we tested its ability to support osteogenic differentiation of stromal stem cells ( MSC) and its suitability for regeneration of craniofa- cial defects. Physiochemical characterizations of the scaffold, including con- tact angle measurements, showed that PVA-PCL-HAB was more hydrophilic than PCL alone or PCL-HAB combined (P < 0.05). Ion release profile studies using ICP-OES of the scaffold showed release of calcium and silica ions, required for initiation of bioactivity. SEM and EDS analyses revealed apatite formation on stimulated body fluid immersed scaffold samples. Culturing human adult dental pulp stem cells (DPSC) and human bone marrow derived MSC seeded on PVA-PCL-HAB scaffold showed enhanced cell proliferation and in vitro osteoblastic differentiation. Cell-containing scaffolds were implanted subcutaneously in immune deficient mice. Histologic ex- amination of retrieved implant sections stained with H&E, Col- lagenType I and Human Vimentin antibody demonstrated that the cells survived in vivo in the implants for at least 8 weeks with evidence of osteoblastic differentiation and angiogenesis within the implants. Our results suggest that PVA-PCL-HAB scaffold can support growth and osteoblast differentiation of MSC and thus should be considered for clinical use in craniofacial tissue regeneration
Original languageEnglish
JournalTissue Engineering. Part A
Volume21
IssueSupplement 1
Pages (from-to)S-58
ISSN1937-3341
DOIs
Publication statusPublished - 2015
Event2015 4th TERMIS World Congress - Boston, United States
Duration: 8 Sep 201511 Sep 2015

Conference

Conference2015 4th TERMIS World Congress
CountryUnited States
CityBoston
Period08/09/201511/09/2015

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