Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?

Cecilie Lærke Glindtvad Tarpø; Menglin Chen; Jens Vinge Nygaard; Lise  Waagensen; Axel Forman; Carl Christian Danielsen; Mehmet Berat Taskin; Karl-Erik Andersson; Susanne Maigaard Axelsen

doi:10.1002/jbm.b.33875

Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?

Cecilie Lærke Glindtvad Tarpø, Menglin Chen, Jens Vinge Nygaard, Lise Waagensen, Axel Forman, Carl Christian Danielsen, Mehmet Berat Taskin, Karl-Erik Andersson, Susanne Maigaard Axelsen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model. Samples were explanted after 4, 8, and 24 weeks, and tested for mechanical properties and the composition of connective tissue. The study showed an increase in mRNA expression for collagen-I (p = 0.0060) and collagen-III (p = 0.0086) in the 4 weeks group with bFGF. The difference was equalized at 8 and 24 weeks. No difference was found at any time for protein amount for collagen-I, collagen-III, and fibronectin. The amount of collagen decreased from 4 to 24 weeks but the fraction of collagen increased. The maximal load of the newly formed tissue showed no effect of bFGF at any time. Exclusively, histology showed a limited ingrowth of collagen fibers after 4 weeks with bFGF but signs of elastin fibers were seen at 24 weeks. The investigation showed that a biodegradable mesh promotes tissue formation with a promising strength. The mesh with bFGF did not represent any advantage on either long or short term in comparison to the mesh without bFGF.

Original language	English
Journal	Journal of Biomedical Materials Research. Part B: Applied Biomaterials
Volume	106
Issue	2
Pages (from-to)	680-688
Number of pages	9
ISSN	1552-4973
DOIs	https://doi.org/10.1002/jbm.b.33875
Publication status	Published - 2018

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10.1002/jbm.b.33875

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Tarpø, C. L. G., Chen, M., Nygaard, J. V., Waagensen, L., Forman, A., Danielsen, C. C., Taskin, M. B., Andersson, K.-E., & Axelsen, S. M. (2018). Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair? Journal of Biomedical Materials Research. Part B: Applied Biomaterials, 106(2), 680-688. https://doi.org/10.1002/jbm.b.33875

@article{4d610203a7214beda30ae5ed1c1f2fe0,

title = "Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?",

abstract = " Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model. Samples were explanted after 4, 8, and 24 weeks, and tested for mechanical properties and the composition of connective tissue. The study showed an increase in mRNA expression for collagen-I (p = 0.0060) and collagen-III (p = 0.0086) in the 4 weeks group with bFGF. The difference was equalized at 8 and 24 weeks. No difference was found at any time for protein amount for collagen-I, collagen-III, and fibronectin. The amount of collagen decreased from 4 to 24 weeks but the fraction of collagen increased. The maximal load of the newly formed tissue showed no effect of bFGF at any time. Exclusively, histology showed a limited ingrowth of collagen fibers after 4 weeks with bFGF but signs of elastin fibers were seen at 24 weeks. The investigation showed that a biodegradable mesh promotes tissue formation with a promising strength. The mesh with bFGF did not represent any advantage on either long or short term in comparison to the mesh without bFGF. ",

author = "Tarp{\o}, {Cecilie L{\ae}rke Glindtvad} and Menglin Chen and Nygaard, {Jens Vinge} and Lise Waagensen and Axel Forman and Danielsen, {Carl Christian} and Taskin, {Mehmet Berat} and Karl-Erik Andersson and Axelsen, {Susanne Maigaard}",

note = "{\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2018",

doi = "10.1002/jbm.b.33875",

language = "English",

volume = "106",

pages = "680--688",

journal = "Journal of Biomedical Materials Research. Part B: Applied Biomaterials",

issn = "1552-4973",

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Tarpø, CLG , Chen, M , Nygaard, JV , Waagensen, L , Forman, A, Danielsen, CC, Taskin, MB, Andersson, K-E & Axelsen, SM 2018, 'Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?', Journal of Biomedical Materials Research. Part B: Applied Biomaterials, vol. 106, no. 2, pp. 680-688. https://doi.org/10.1002/jbm.b.33875

Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair? / Tarpø, Cecilie Lærke Glindtvad ; Chen, Menglin ; Nygaard, Jens Vinge et al.
In: Journal of Biomedical Materials Research. Part B: Applied Biomaterials, Vol. 106, No. 2, 2018, p. 680-688.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model

T2 - A possible treatment for pelvic floor repair?

AU - Tarpø, Cecilie Lærke Glindtvad

AU - Chen, Menglin

AU - Nygaard, Jens Vinge

AU - Waagensen, Lise

AU - Forman, Axel

AU - Danielsen, Carl Christian

AU - Taskin, Mehmet Berat

AU - Andersson, Karl-Erik

AU - Axelsen, Susanne Maigaard

PY - 2018

Y1 - 2018

N2 - Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model. Samples were explanted after 4, 8, and 24 weeks, and tested for mechanical properties and the composition of connective tissue. The study showed an increase in mRNA expression for collagen-I (p = 0.0060) and collagen-III (p = 0.0086) in the 4 weeks group with bFGF. The difference was equalized at 8 and 24 weeks. No difference was found at any time for protein amount for collagen-I, collagen-III, and fibronectin. The amount of collagen decreased from 4 to 24 weeks but the fraction of collagen increased. The maximal load of the newly formed tissue showed no effect of bFGF at any time. Exclusively, histology showed a limited ingrowth of collagen fibers after 4 weeks with bFGF but signs of elastin fibers were seen at 24 weeks. The investigation showed that a biodegradable mesh promotes tissue formation with a promising strength. The mesh with bFGF did not represent any advantage on either long or short term in comparison to the mesh without bFGF.

AB - Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model. Samples were explanted after 4, 8, and 24 weeks, and tested for mechanical properties and the composition of connective tissue. The study showed an increase in mRNA expression for collagen-I (p = 0.0060) and collagen-III (p = 0.0086) in the 4 weeks group with bFGF. The difference was equalized at 8 and 24 weeks. No difference was found at any time for protein amount for collagen-I, collagen-III, and fibronectin. The amount of collagen decreased from 4 to 24 weeks but the fraction of collagen increased. The maximal load of the newly formed tissue showed no effect of bFGF at any time. Exclusively, histology showed a limited ingrowth of collagen fibers after 4 weeks with bFGF but signs of elastin fibers were seen at 24 weeks. The investigation showed that a biodegradable mesh promotes tissue formation with a promising strength. The mesh with bFGF did not represent any advantage on either long or short term in comparison to the mesh without bFGF.

U2 - 10.1002/jbm.b.33875

DO - 10.1002/jbm.b.33875

M3 - Journal article

C2 - 28306194

SN - 1552-4973

VL - 106

SP - 680

EP - 688

JO - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

JF - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

IS - 2

ER -