Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs

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CRISPR/Cas9 is an effective and easy-to-use tool for editing the genome of many human cancer cell lines. However, in some hard-to-transfect cell lines and primary cells, gene editing is more challenging. This protocol details an electroporation-based protocol for the delivery of Cas9 protein from Streptococcus pyogenes complexed with chemically modified sgRNAs. We have found this protocol to work very efficiently in numerous cell lines and primary cells that are difficult to transfect by conventional chemical-based transfection methods.

Original languageEnglish
Title of host publicationCRISPR gene editing : Methods and protocols
EditorsYonglun Luo
Number of pages8
Place of publicationNew York
PublisherSpringer Science+Business Media
Publication year2019
Pages127-134
ISBN (print)978-1-4939-9169-3
ISBN (Electronic)978-1-4939-9170-9
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume1961
ISSN1064-3745

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