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Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters

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Cellular and brain metabolism of dopamine can be
correlated with a number of neurodegenerative disorders, and as such,
in vivo analysis of dopamine in the presence of structurally related
neurotransmitters (NT) represents a holy grail of neuroscience.
Interference from those NTs generally does not allow selective
electroanalysis of dopamine, which redox transformation overlaps with
those of other catecholamines. In our previous work, we reported an
electrochemical RNA-aptamer-based biosensor for specific analysis of
dopamine (Analytical Chemistry, 2013; Vol. 85, p 121). However, the
overall design of the biosensor restricted its stability and impeded its
operation in serum. Here, we show that specific biorecognition and
electroanalysis of dopamine in serum can be performed by the RNA
aptamer tethered to cysteamine-modified gold electrodes via the
alkanethiol linker. The stabilized dopamine aptasensor allowed
continuous 20 h amperometric analysis of dopamine in 10% serum within the physiologically important 0.11 μM range
and in the presence of catechol and such dopamine precursors and metabolites as norepinephrine and L-DOPA. In a flowinjection
mode, the aptasensor response to dopamine was ∼1 s, the sensitivity of analysis, optimized by adjusting the aptamer
surface coverage, was 67 ± 1 nA μM−1 cm−2, and the dopamine LOD was 62 nM. The proposed design of the aptasensor,
exploiting both the aptamer alkanethiol tethering to the electrode and screening of the catecholamine-aptamer electrostatic
interactions, allows direct monitoring of dopamine levels in biological fluids in the presence of competitive NT and thus may be
further applicable in biomedical research.
Original languageEnglish
JournalAnalytical Chemistry
Pages (from-to)3608-3616
Number of pages9
Publication statusPublished - 2016

    Research areas

  • DOPAMINE, Electrochemistry, SERUM

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