Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

Maria Vad Jakobsen; Anne Louise Askou; Karin Stenderup Dokkedahl; Cecilia Rosada Kjeldsen; Frederik Dagnæs-Hansen; Thomas G. Jensen; Thomas Juhl Corydon; Jacob Giehm Mikkelsen; Lars Aagaard

Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

Maria Vad Jakobsen, Anne Louise Askou, Karin Stenderup Dokkedahl, Cecilia Rosada Kjeldsen, Frederik Dagnæs-Hansen, Thomas G. Jensen, Thomas Juhl Corydon, Jacob Giehm Mikkelsen, Lars Aagaard

Research output: Contribution to conference › Poster › Research › peer-review

Abstract

Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimaging
demonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

Original language	English
Publication date	18 Sept 2015
Number of pages	1
Publication status	Published - 18 Sept 2015
Event	Annual Congress The European Society of Gene and Cell Therapy (ESGCT) - Finlandia Hall, Helsinki, Finland Duration: 17 Sept 2015 → 20 Sept 2015

Conference

Conference	Annual Congress The European Society of Gene and Cell Therapy (ESGCT)
Location	Finlandia Hall
Country/Territory	Finland
City	Helsinki
Period	17/09/2015 → 20/09/2015

Cite this

Jakobsen, M. V., Askou, A. L., Dokkedahl, K. S., Kjeldsen, C. R., Dagnæs-Hansen, F., Jensen, T. G., Corydon, T. J., Mikkelsen, J. G., & Aagaard, L. (2015). Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. Poster session presented at Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

@conference{0fbb2634142c49409221d13a914d8035,

title = "Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery",

abstract = "Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.",

author = "Jakobsen, {Maria Vad} and Askou, {Anne Louise} and Dokkedahl, {Karin Stenderup} and Kjeldsen, {Cecilia Rosada} and Frederik Dagn{\ae}s-Hansen and Jensen, {Thomas G.} and Corydon, {Thomas Juhl} and Mikkelsen, {Jacob Giehm} and Lars Aagaard",

year = "2015",

month = sep,

day = "18",

language = "English",

note = "Annual Congress The European Society of Gene and Cell Therapy (ESGCT) ; Conference date: 17-09-2015 Through 20-09-2015",

}

Jakobsen, MV, Askou, AL , Dokkedahl, KS, Kjeldsen, CR, Dagnæs-Hansen, F, Jensen, TG , Corydon, TJ , Mikkelsen, JG & Aagaard, L 2015, 'Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery', Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland, 17/09/2015 - 20/09/2015.

Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. / Jakobsen, Maria Vad; Askou, Anne Louise ; Dokkedahl, Karin Stenderup et al.
2015. Poster session presented at Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

Research output: Contribution to conference › Poster › Research › peer-review

TY - CONF

T1 - Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

AU - Jakobsen, Maria Vad

AU - Askou, Anne Louise

AU - Dokkedahl, Karin Stenderup

AU - Kjeldsen, Cecilia Rosada

AU - Dagnæs-Hansen, Frederik

AU - Jensen, Thomas G.

AU - Corydon, Thomas Juhl

AU - Mikkelsen, Jacob Giehm

AU - Aagaard, Lars

PY - 2015/9/18

Y1 - 2015/9/18

N2 - Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

AB - Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

UR - http://www.esgct.eu/

M3 - Poster

T2 - Annual Congress The European Society of Gene and Cell Therapy (ESGCT)

Y2 - 17 September 2015 through 20 September 2015

ER -

Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

Abstract

Conference

Other files and links

Cite this