Efficient data acquisition with three-channel centerpieces in sedimentation velocity

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DOI

  • Kristian Juul-Madsen
  • Huaying Zhao, Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
  • ,
  • Thomas Vorup-Jensen
  • Peter Schuck, Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. Electronic address: Peter.Schuck@nih.gov.

Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.

Original languageEnglish
Article number113414
JournalAnalytical Biochemistry
Volume586
Pages (from-to)113414
ISSN0003-2697
DOIs
Publication statusPublished - 1 Dec 2019

    Research areas

  • 3D printing, Analytical ultracentrifugation, Laboratory automation, Sedimentation velocity

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