Efficient data acquisition with three-channel centerpieces in sedimentation velocity

Kristian Juul-Madsen; Huaying Zhao; Thomas Vorup-Jensen; Peter Schuck

doi:10.1016/j.ab.2019.113414

Efficient data acquisition with three-channel centerpieces in sedimentation velocity

Kristian Juul-Madsen, Huaying Zhao, Thomas Vorup-Jensen, Peter Schuck

Department of Biomedicine - Forskning og uddannelse, Skou-bygningen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

5 Citations (Scopus)

Abstract

Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.

Original language	English
Article number	113414
Journal	Analytical Biochemistry
Volume	586
Pages (from-to)	113414
ISSN	0003-2697
DOIs	https://doi.org/10.1016/j.ab.2019.113414
Publication status	Published - 1 Dec 2019

Keywords

3D printing
Analytical ultracentrifugation
Laboratory automation
Sedimentation velocity

Access to Document

10.1016/j.ab.2019.113414

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768728/pdf/nihms-1539915.pdf

Cite this

@article{448e9c4f30664a4899e1f81f614f2716,

title = "Efficient data acquisition with three-channel centerpieces in sedimentation velocity",

abstract = "Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.",

keywords = "Ultracentrifugation, 3D printing, Analytical ultracentrifugation, Laboratory automation, Sedimentation velocity",

author = "Kristian Juul-Madsen and Huaying Zhao and Thomas Vorup-Jensen and Peter Schuck",

note = "Copyright {\textcopyright} 2019. Published by Elsevier Inc.",

year = "2019",

month = dec,

day = "1",

doi = "10.1016/j.ab.2019.113414",

language = "English",

volume = "586",

pages = "113414",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

Efficient data acquisition with three-channel centerpieces in sedimentation velocity. / Juul-Madsen, Kristian; Zhao, Huaying; Vorup-Jensen, Thomas et al.
In: Analytical Biochemistry, Vol. 586, 113414, 01.12.2019, p. 113414.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Efficient data acquisition with three-channel centerpieces in sedimentation velocity

AU - Juul-Madsen, Kristian

AU - Zhao, Huaying

AU - Vorup-Jensen, Thomas

AU - Schuck, Peter

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.

AB - Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.

KW - Ultracentrifugation

KW - 3D printing

KW - Analytical ultracentrifugation

KW - Laboratory automation

KW - Sedimentation velocity

U2 - 10.1016/j.ab.2019.113414

DO - 10.1016/j.ab.2019.113414

M3 - Journal article

C2 - 31493371

SN - 0003-2697

VL - 586

SP - 113414

JO - Analytical Biochemistry

JF - Analytical Biochemistry

M1 - 113414

ER -

Efficient data acquisition with three-channel centerpieces in sedimentation velocity

Abstract

Keywords

Access to Document

Fingerprint

Cite this