Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability

Mirka Thorsteinsson; Martin Riis Weisbjerg; Peter Lund; Marco Battelli; Élisabeth Chassé; Annette Bruhn; Mette Olaf Nielsen

doi:10.1016/j.algal.2023.103139

Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability

Mirka Thorsteinsson^*, Martin Riis Weisbjerg, Peter Lund, Marco Battelli, Élisabeth Chassé, Annette Bruhn, Mette Olaf Nielsen

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Enteric methane (CH₄) from cattle constitutes a large proportion of the emission of anthropogenic greenhouse gasses. The objectives of this study were to investigate interspecies and seasonal variations in macroalgae, procured from the Northern Hemisphere, on CH₄ production and rumen feed degradability in vitro. Corn silage or grass/clover silage (0.5 g), were incubated separately in buffered rumen fluid with addition of one of 22 different macroalgae (0.1 g), including both red, green, and brown species, hereof four brown species harvested at different seasons, resulting in an inclusion rate of 17 %. Rumen degradability of the pure macroalgae was determined by incubating macroalgae (0.5 g) without any basal feed. Accumulated gas production during 48 h of incubation was determined using the Ankom^RF system equipped with airtight gasbags. Basal feeds without macroalgae addition served as controls, while the red macroalgae Asparagopsis taxiformis was included as a methane-inhibiting control. Each sample type was tested in triplicate in two separate runs. Amount of undegraded dry matter (DM) and organic matter (OM) after incubation was determined by filtration. Gas composition and concentration of volatile fatty acids (VFA) were determined by gas chromatography. Both A. taxiformis and Fucus serratus significantly reduced CH₄ production, expressed per g DM, OM, degraded DM, and OM, when added to corn silage. Similar CH₄ mitigating effect was observed when A. taxiformis was added to grass/clover silage, while F. serratus only significantly reduced the CH₄ production expressed per g DM. None of the species included in the investigation of seasonal variations decreased CH₄ production compared to the basal feeds. Palmaria palmata had the highest rumen degradability and associated VFA production, while F. vesiculosus and F. serratus had the numerically lowest rumen degradability and VFA production, respectively. In conclusion, F. serratus was the only Northern procurable macroalga showing CH₄ mitigating potential, which depended on the nature of the basal feed.

Original language	English
Article number	103139
Journal	Algal Research
Volume	73
ISSN	2211-9264
DOIs	https://doi.org/10.1016/j.algal.2023.103139
Publication status	Published - Jun 2023

Keywords

Alternative feedstuffs
Cattle
Chemical composition
Fucus serratus
Rumen fermentation
Seaweed

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10.1016/j.algal.2023.103139

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Thorsteinsson, M., Weisbjerg, M. R., Lund, P., Battelli, M., Chassé, É., Bruhn, A., & Nielsen, M. O. (2023). Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability. Algal Research, 73, Article 103139. https://doi.org/10.1016/j.algal.2023.103139

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title = "Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability",

abstract = "Enteric methane (CH4) from cattle constitutes a large proportion of the emission of anthropogenic greenhouse gasses. The objectives of this study were to investigate interspecies and seasonal variations in macroalgae, procured from the Northern Hemisphere, on CH4 production and rumen feed degradability in vitro. Corn silage or grass/clover silage (0.5 g), were incubated separately in buffered rumen fluid with addition of one of 22 different macroalgae (0.1 g), including both red, green, and brown species, hereof four brown species harvested at different seasons, resulting in an inclusion rate of 17 %. Rumen degradability of the pure macroalgae was determined by incubating macroalgae (0.5 g) without any basal feed. Accumulated gas production during 48 h of incubation was determined using the AnkomRF system equipped with airtight gasbags. Basal feeds without macroalgae addition served as controls, while the red macroalgae Asparagopsis taxiformis was included as a methane-inhibiting control. Each sample type was tested in triplicate in two separate runs. Amount of undegraded dry matter (DM) and organic matter (OM) after incubation was determined by filtration. Gas composition and concentration of volatile fatty acids (VFA) were determined by gas chromatography. Both A. taxiformis and Fucus serratus significantly reduced CH4 production, expressed per g DM, OM, degraded DM, and OM, when added to corn silage. Similar CH4 mitigating effect was observed when A. taxiformis was added to grass/clover silage, while F. serratus only significantly reduced the CH4 production expressed per g DM. None of the species included in the investigation of seasonal variations decreased CH4 production compared to the basal feeds. Palmaria palmata had the highest rumen degradability and associated VFA production, while F. vesiculosus and F. serratus had the numerically lowest rumen degradability and VFA production, respectively. In conclusion, F. serratus was the only Northern procurable macroalga showing CH4 mitigating potential, which depended on the nature of the basal feed.",

keywords = "Alternative feedstuffs, Cattle, Chemical composition, Fucus serratus, Rumen fermentation, Seaweed",

author = "Mirka Thorsteinsson and Weisbjerg, {Martin Riis} and Peter Lund and Marco Battelli and {\'E}lisabeth Chass{\'e} and Annette Bruhn and Nielsen, {Mette Olaf}",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = jun,

doi = "10.1016/j.algal.2023.103139",

language = "English",

volume = "73",

journal = "Algal Research",

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Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability. / Thorsteinsson, Mirka ; Weisbjerg, Martin Riis ; Lund, Peter et al.
In: Algal Research, Vol. 73, 103139, 06.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability

AU - Thorsteinsson, Mirka

AU - Weisbjerg, Martin Riis

AU - Lund, Peter

AU - Battelli, Marco

AU - Chassé, Élisabeth

AU - Bruhn, Annette

AU - Nielsen, Mette Olaf

PY - 2023/6

Y1 - 2023/6

N2 - Enteric methane (CH4) from cattle constitutes a large proportion of the emission of anthropogenic greenhouse gasses. The objectives of this study were to investigate interspecies and seasonal variations in macroalgae, procured from the Northern Hemisphere, on CH4 production and rumen feed degradability in vitro. Corn silage or grass/clover silage (0.5 g), were incubated separately in buffered rumen fluid with addition of one of 22 different macroalgae (0.1 g), including both red, green, and brown species, hereof four brown species harvested at different seasons, resulting in an inclusion rate of 17 %. Rumen degradability of the pure macroalgae was determined by incubating macroalgae (0.5 g) without any basal feed. Accumulated gas production during 48 h of incubation was determined using the AnkomRF system equipped with airtight gasbags. Basal feeds without macroalgae addition served as controls, while the red macroalgae Asparagopsis taxiformis was included as a methane-inhibiting control. Each sample type was tested in triplicate in two separate runs. Amount of undegraded dry matter (DM) and organic matter (OM) after incubation was determined by filtration. Gas composition and concentration of volatile fatty acids (VFA) were determined by gas chromatography. Both A. taxiformis and Fucus serratus significantly reduced CH4 production, expressed per g DM, OM, degraded DM, and OM, when added to corn silage. Similar CH4 mitigating effect was observed when A. taxiformis was added to grass/clover silage, while F. serratus only significantly reduced the CH4 production expressed per g DM. None of the species included in the investigation of seasonal variations decreased CH4 production compared to the basal feeds. Palmaria palmata had the highest rumen degradability and associated VFA production, while F. vesiculosus and F. serratus had the numerically lowest rumen degradability and VFA production, respectively. In conclusion, F. serratus was the only Northern procurable macroalga showing CH4 mitigating potential, which depended on the nature of the basal feed.

AB - Enteric methane (CH4) from cattle constitutes a large proportion of the emission of anthropogenic greenhouse gasses. The objectives of this study were to investigate interspecies and seasonal variations in macroalgae, procured from the Northern Hemisphere, on CH4 production and rumen feed degradability in vitro. Corn silage or grass/clover silage (0.5 g), were incubated separately in buffered rumen fluid with addition of one of 22 different macroalgae (0.1 g), including both red, green, and brown species, hereof four brown species harvested at different seasons, resulting in an inclusion rate of 17 %. Rumen degradability of the pure macroalgae was determined by incubating macroalgae (0.5 g) without any basal feed. Accumulated gas production during 48 h of incubation was determined using the AnkomRF system equipped with airtight gasbags. Basal feeds without macroalgae addition served as controls, while the red macroalgae Asparagopsis taxiformis was included as a methane-inhibiting control. Each sample type was tested in triplicate in two separate runs. Amount of undegraded dry matter (DM) and organic matter (OM) after incubation was determined by filtration. Gas composition and concentration of volatile fatty acids (VFA) were determined by gas chromatography. Both A. taxiformis and Fucus serratus significantly reduced CH4 production, expressed per g DM, OM, degraded DM, and OM, when added to corn silage. Similar CH4 mitigating effect was observed when A. taxiformis was added to grass/clover silage, while F. serratus only significantly reduced the CH4 production expressed per g DM. None of the species included in the investigation of seasonal variations decreased CH4 production compared to the basal feeds. Palmaria palmata had the highest rumen degradability and associated VFA production, while F. vesiculosus and F. serratus had the numerically lowest rumen degradability and VFA production, respectively. In conclusion, F. serratus was the only Northern procurable macroalga showing CH4 mitigating potential, which depended on the nature of the basal feed.

KW - Alternative feedstuffs

KW - Cattle

KW - Chemical composition

KW - Fucus serratus

KW - Rumen fermentation

KW - Seaweed

U2 - 10.1016/j.algal.2023.103139

DO - 10.1016/j.algal.2023.103139

M3 - Journal article

AN - SCOPUS:85161292693

SN - 2211-9264

VL - 73

JO - Algal Research

JF - Algal Research

M1 - 103139

ER -

Effects of seasonal and interspecies differences in macroalgae procured from temperate seas on the Northern hemisphere on in vitro methane mitigating properties and rumen degradability

Abstract

Keywords

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