Effects of anesthesia on [11C]raclopride binding in the rat brain

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  • Positron Emission Tomography Center
Background
Very often rats are anesthetized prior to micro positron emission tomography (microPET) brain imaging in order to prevent head movements. Anesthesia can be administered by inhalation agents, such as isoflurane, or injection mixtures, such as fentanyl-fluanisone-midazolam. Unfortunately, anesthesia affects a variety of physiological variables, including in the brain.

Aim
The aim of this study was to compare the effects of inhalation and injection anesthesia on the binding potential of the dopaminergic D2/3 tracer [11C]raclopride used for PET brain imaging in human and animal studies.

Materials & Methods
Nine male Lew/Mol rats were assigned to either inhalation (isoflurane; N=4) or injection (fentanyl-fluanisone-midazolam; N=5) anesthesia. Catheters were surgically placed in femoral arteries and veins for blood sampling and tracer injection. After a short attenuation scan, the rats were PET scanned for 90 minutes after injection of [11C]raclopride.

Results
We found that rats anesthetized with isoflurane had double the binding potential in the striatum compared with fentanyl-fluanisone-midazolam anesthetized rats.


Conclusion
Our results are in agreement with other studies showing that anesthesia may have a major influence on brain imaging studies involving tracer kinetics in rats and other laboratory animals.

Original languageEnglish
Publication year17 Jun 2011
Publication statusPublished - 17 Jun 2011
EventOAK Meeting - Århus, Denmark
Duration: 17 Dec 2010 → …

Conference

ConferenceOAK Meeting
CountryDenmark
CityÅrhus
Period17/12/2010 → …

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