Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon

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Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon. / Autrup, Herman; Harris, Curtis C.; Fugaro, Steven; Selkirk, James K.

In: Chemico-Biological Interactions, Vol. 18, No. 3, 09.1977, p. 337-347.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Autrup, H, Harris, CC, Fugaro, S & Selkirk, JK 1977, 'Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon', Chemico-Biological Interactions, vol. 18, no. 3, pp. 337-347. https://doi.org/10.1016/0009-2797(77)90019-9

APA

Autrup, H., Harris, C. C., Fugaro, S., & Selkirk, J. K. (1977). Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon. Chemico-Biological Interactions, 18(3), 337-347. https://doi.org/10.1016/0009-2797(77)90019-9

CBE

MLA

Vancouver

Autrup H, Harris CC, Fugaro S, Selkirk JK. Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon. Chemico-Biological Interactions. 1977 Sep;18(3):337-347. https://doi.org/10.1016/0009-2797(77)90019-9

Author

Autrup, Herman ; Harris, Curtis C. ; Fugaro, Steven ; Selkirk, James K. / Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon. In: Chemico-Biological Interactions. 1977 ; Vol. 18, No. 3. pp. 337-347.

Bibtex

@article{a24f70f001e711dbbee902004c4f4f50,
title = "Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon",
abstract = "The effect of various co- and anti-carcinogens of colon carcinogenesis on the metabolism of benzo(a)pyrene (BP) in cultured rat colon is reported. Rat colon enzymatically converted BP into metabolites which bind to cellular macromolecules i.e., DNA and protein. Activity of aryl hydrocarbon hydroxylase (AHH) activity and binding levels of BP to macromolecules were higher in the descending colon when compared to other segments. The major metabolites of BP, extractable with ethylacetate, were quinones, tetrols, 7,8-diol and a peak containing 9,10-dihydroxy-9,10-dihydrobenzo(a)pyrene and 7,8,9-trihydroxy-7,8-dihydrobenzo(a)pyrene. The binding levels of BP to DNA and protein in the explant was lowered by co-incubation with 7,8-benzoflavone (7,8-BF) (3.6 and 18.0 micron), a known inhibitor of AHH, and with disulfiram (100 micron), an anti-oxidant. The absence of vitamin A in the media also resulted in a lower level of BP binding to DNA and protein and in lower activity of AHH. Pretreatment with known inducers of AHH such as phenobarbital (PB) or benz(a)anthracene (BA), did not have any significant effect on the binding levels of BP to DNA or on the AHH activity. Of the bile acids investigated only taurodeoxycholic acid significantly increased the binding level of BP to DNA.",
author = "Herman Autrup and Harris, {Curtis C.} and Steven Fugaro and Selkirk, {James K.}",
year = "1977",
month = "9",
doi = "10.1016/0009-2797(77)90019-9",
language = "English",
volume = "18",
pages = "337--347",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd.",
number = "3",

}

RIS

TY - JOUR

T1 - Effect of various chemicals on the metabolism of benzo(a)pyrene by cultured rat colon

AU - Autrup, Herman

AU - Harris, Curtis C.

AU - Fugaro, Steven

AU - Selkirk, James K.

PY - 1977/9

Y1 - 1977/9

N2 - The effect of various co- and anti-carcinogens of colon carcinogenesis on the metabolism of benzo(a)pyrene (BP) in cultured rat colon is reported. Rat colon enzymatically converted BP into metabolites which bind to cellular macromolecules i.e., DNA and protein. Activity of aryl hydrocarbon hydroxylase (AHH) activity and binding levels of BP to macromolecules were higher in the descending colon when compared to other segments. The major metabolites of BP, extractable with ethylacetate, were quinones, tetrols, 7,8-diol and a peak containing 9,10-dihydroxy-9,10-dihydrobenzo(a)pyrene and 7,8,9-trihydroxy-7,8-dihydrobenzo(a)pyrene. The binding levels of BP to DNA and protein in the explant was lowered by co-incubation with 7,8-benzoflavone (7,8-BF) (3.6 and 18.0 micron), a known inhibitor of AHH, and with disulfiram (100 micron), an anti-oxidant. The absence of vitamin A in the media also resulted in a lower level of BP binding to DNA and protein and in lower activity of AHH. Pretreatment with known inducers of AHH such as phenobarbital (PB) or benz(a)anthracene (BA), did not have any significant effect on the binding levels of BP to DNA or on the AHH activity. Of the bile acids investigated only taurodeoxycholic acid significantly increased the binding level of BP to DNA.

AB - The effect of various co- and anti-carcinogens of colon carcinogenesis on the metabolism of benzo(a)pyrene (BP) in cultured rat colon is reported. Rat colon enzymatically converted BP into metabolites which bind to cellular macromolecules i.e., DNA and protein. Activity of aryl hydrocarbon hydroxylase (AHH) activity and binding levels of BP to macromolecules were higher in the descending colon when compared to other segments. The major metabolites of BP, extractable with ethylacetate, were quinones, tetrols, 7,8-diol and a peak containing 9,10-dihydroxy-9,10-dihydrobenzo(a)pyrene and 7,8,9-trihydroxy-7,8-dihydrobenzo(a)pyrene. The binding levels of BP to DNA and protein in the explant was lowered by co-incubation with 7,8-benzoflavone (7,8-BF) (3.6 and 18.0 micron), a known inhibitor of AHH, and with disulfiram (100 micron), an anti-oxidant. The absence of vitamin A in the media also resulted in a lower level of BP binding to DNA and protein and in lower activity of AHH. Pretreatment with known inducers of AHH such as phenobarbital (PB) or benz(a)anthracene (BA), did not have any significant effect on the binding levels of BP to DNA or on the AHH activity. Of the bile acids investigated only taurodeoxycholic acid significantly increased the binding level of BP to DNA.

U2 - 10.1016/0009-2797(77)90019-9

DO - 10.1016/0009-2797(77)90019-9

M3 - Journal article

VL - 18

SP - 337

EP - 347

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

IS - 3

ER -