Effect of Sample Storage Conditions on Measurements of Salivary Cotinine Levels

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Effect of Sample Storage Conditions on Measurements of Salivary Cotinine Levels. / Manzolli Leite, Fabio Renato; Bælum, Vibeke; Pajaniaye, Julie et al.

In: Metabolites, Vol. 10, No. 9, 365, 2020.

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@article{add8311eaf8a49c7852097b05746a438,
title = "Effect of Sample Storage Conditions on Measurements of Salivary Cotinine Levels",
abstract = "Abstract: Information on smoking exposure obtained with self-reports may be inaccurate. Cotininehas a large half-life and its salivary levels correlate well with plasmatic levels. The influence of storageconditions on the validity and precision of salivary cotinine assessments has rarely been evaluated.Here, smokers donated saliva samples, which were sent for immediate analysis, mail posting, storageat 4 ◦C for 30 or 90 days, or storage at −20 ◦C for 30 or 90 days. Cotinine levels were determined usingenzyme-linked immune-sorbent assay. Agreement of cotinine level measurements was assessed usingBland-Altman analyses. Average age (years), duration of smoking (years) and number of cigarettessmoked (/day) were 55.4 (±SD 9.4), 35.1 (±SD 11.3), and 15.3 (±SD 7.6). The mean immediate cotininelevel was 457 ng/mL (range 11.3 to 1318 ng/mL). Mean cotinine levels in samples analyzed afterdelay ranged between 433 ng/mL (−20 ◦C 30 days) and 468 ng/mL (4 ◦C 30 days). A dose-responsegradient was observed in the relationship between salivary cotinine level and self-reported smokingstatus. A good agreement between cotinine levels for all storage conditions compared with immediateanalysis was observed, with average differences ranging from −11 to 24 ng/mL. Cotinine levelsremained stable regardless of the tested condition. The stability of salivary cotinine may enablesamples to be obtained in difficult-to-reach areas, reduce study costs, and improve the validity of theinformation on exposure to smoking.",
author = "{Manzolli Leite}, {Fabio Renato} and Vibeke B{\ae}lum and Julie Pajaniaye and Abildtrup, {Lisbeth Ann} and Rodrigo Lopez",
year = "2020",
doi = "10.3390/metabo10090365",
language = "English",
volume = "10",
journal = "Metabolites",
issn = "2218-1989",
publisher = "M D P I AG",
number = "9",

}

RIS

TY - JOUR

T1 - Effect of Sample Storage Conditions on Measurements of Salivary Cotinine Levels

AU - Manzolli Leite, Fabio Renato

AU - Bælum, Vibeke

AU - Pajaniaye, Julie

AU - Abildtrup, Lisbeth Ann

AU - Lopez, Rodrigo

PY - 2020

Y1 - 2020

N2 - Abstract: Information on smoking exposure obtained with self-reports may be inaccurate. Cotininehas a large half-life and its salivary levels correlate well with plasmatic levels. The influence of storageconditions on the validity and precision of salivary cotinine assessments has rarely been evaluated.Here, smokers donated saliva samples, which were sent for immediate analysis, mail posting, storageat 4 ◦C for 30 or 90 days, or storage at −20 ◦C for 30 or 90 days. Cotinine levels were determined usingenzyme-linked immune-sorbent assay. Agreement of cotinine level measurements was assessed usingBland-Altman analyses. Average age (years), duration of smoking (years) and number of cigarettessmoked (/day) were 55.4 (±SD 9.4), 35.1 (±SD 11.3), and 15.3 (±SD 7.6). The mean immediate cotininelevel was 457 ng/mL (range 11.3 to 1318 ng/mL). Mean cotinine levels in samples analyzed afterdelay ranged between 433 ng/mL (−20 ◦C 30 days) and 468 ng/mL (4 ◦C 30 days). A dose-responsegradient was observed in the relationship between salivary cotinine level and self-reported smokingstatus. A good agreement between cotinine levels for all storage conditions compared with immediateanalysis was observed, with average differences ranging from −11 to 24 ng/mL. Cotinine levelsremained stable regardless of the tested condition. The stability of salivary cotinine may enablesamples to be obtained in difficult-to-reach areas, reduce study costs, and improve the validity of theinformation on exposure to smoking.

AB - Abstract: Information on smoking exposure obtained with self-reports may be inaccurate. Cotininehas a large half-life and its salivary levels correlate well with plasmatic levels. The influence of storageconditions on the validity and precision of salivary cotinine assessments has rarely been evaluated.Here, smokers donated saliva samples, which were sent for immediate analysis, mail posting, storageat 4 ◦C for 30 or 90 days, or storage at −20 ◦C for 30 or 90 days. Cotinine levels were determined usingenzyme-linked immune-sorbent assay. Agreement of cotinine level measurements was assessed usingBland-Altman analyses. Average age (years), duration of smoking (years) and number of cigarettessmoked (/day) were 55.4 (±SD 9.4), 35.1 (±SD 11.3), and 15.3 (±SD 7.6). The mean immediate cotininelevel was 457 ng/mL (range 11.3 to 1318 ng/mL). Mean cotinine levels in samples analyzed afterdelay ranged between 433 ng/mL (−20 ◦C 30 days) and 468 ng/mL (4 ◦C 30 days). A dose-responsegradient was observed in the relationship between salivary cotinine level and self-reported smokingstatus. A good agreement between cotinine levels for all storage conditions compared with immediateanalysis was observed, with average differences ranging from −11 to 24 ng/mL. Cotinine levelsremained stable regardless of the tested condition. The stability of salivary cotinine may enablesamples to be obtained in difficult-to-reach areas, reduce study costs, and improve the validity of theinformation on exposure to smoking.

U2 - 10.3390/metabo10090365

DO - 10.3390/metabo10090365

M3 - Journal article

C2 - 32911758

VL - 10

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 9

M1 - 365

ER -