Abstract
Aim:
Pea protein isolates are increasingly employed as functional ingredients in food products. However, commercial pea protein isolates still show low solubility and varying functional properties. It is hypothesized that better control of the extraction conditions may be a suitable strategy to improve the quality of pea protein ingredients. The objective of this work was to understand the influence of the ionic strength during the extraction conditions on pea protein composition, structure, and functionality.
Method:
The solubility, structure, composition, and colloidal behavior of pea proteins extracted at different ionic strengths (0.0 – 1.2 M NaCl) were studied, using hydration radius analysis, fluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), size exclusion chromatography with multi-angle static light scattering and refractive index (SEC-MALS-RI). The interfacial behavior was studied with drop tensiometer.
Results:
Solubility and size of the protein aggregates signific
Pea protein isolates are increasingly employed as functional ingredients in food products. However, commercial pea protein isolates still show low solubility and varying functional properties. It is hypothesized that better control of the extraction conditions may be a suitable strategy to improve the quality of pea protein ingredients. The objective of this work was to understand the influence of the ionic strength during the extraction conditions on pea protein composition, structure, and functionality.
Method:
The solubility, structure, composition, and colloidal behavior of pea proteins extracted at different ionic strengths (0.0 – 1.2 M NaCl) were studied, using hydration radius analysis, fluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), size exclusion chromatography with multi-angle static light scattering and refractive index (SEC-MALS-RI). The interfacial behavior was studied with drop tensiometer.
Results:
Solubility and size of the protein aggregates signific
| Original language | English |
|---|---|
| Publication date | 2022 |
| Publication status | Published - 2022 |
| Event | 36th EFFoST International Conference - Dublin Duration: 7 Nov 2022 → 9 Nov 2022 |
Conference
| Conference | 36th EFFoST International Conference |
|---|---|
| City | Dublin |
| Period | 07/11/2022 → 09/11/2022 |