TY - JOUR
T1 - Effect of micronization and vitamin E supplementation on ruminal biohydrogenation kinetic of whole flaked rapeseed
AU - Zarnegar, Zohreh
AU - Lashkari, Saman
AU - Ebrahimi, Seyed Hadi
AU - Valizadeh, Reza
AU - Jensen, Søren Krogh
AU - Naserian, Abbas Ali
PY - 2024
Y1 - 2024
N2 - The effect of heat treatment (raw or micronization) and vitamin E supplementation (0 or 200 mg/kg DM of RRR-α-tocopherol) on ruminal biohydrogenation (BH) kinetic of whole flaked rapeseed was studied using in vitro batch culture in a 2 × 2 factorial design. Experimental treatments were: whole flaked raw rapeseed without vitamin E (RR
0), whole flaked raw rapeseed supplemented with 200 mg/kg DM of vitamin E (RR
200), micronized whole flaked rapeseed without vitamin E (MR
0) and micronized whole flaked rapeseed supplemented with 200 mg/kg DM of vitamin E (MR
200). The disappearance of linoleic acid (LA), alpha-linolenic acid (LnA), oleic acid (OA), and appearance of stearic acid (SA), and C18:1 trans-11 (VA) were significantly decreased by micronization (P < 0.0001 for all). The appearance of cis-9, trans-11 conjugated linoleic acid (CLA; P < 0.0001) and C18:1 trans-10 (P = 0.01) was significantly increased by micronization. Biohydrogenation of LA (P = 0.03) and OA (P = 0.02) was significantly decreased by vitamin E. There was a tendency to reduce disappearance of LnA by vitamin E (P = 0.06). In conclusion, micronization is an effective method to protect unsaturated fatty acids (UFA) from ruminal BH. Vitamin E supplementation can be an advantageous strategy to decrease LA, LnA and OA biohydrogenation.
AB - The effect of heat treatment (raw or micronization) and vitamin E supplementation (0 or 200 mg/kg DM of RRR-α-tocopherol) on ruminal biohydrogenation (BH) kinetic of whole flaked rapeseed was studied using in vitro batch culture in a 2 × 2 factorial design. Experimental treatments were: whole flaked raw rapeseed without vitamin E (RR
0), whole flaked raw rapeseed supplemented with 200 mg/kg DM of vitamin E (RR
200), micronized whole flaked rapeseed without vitamin E (MR
0) and micronized whole flaked rapeseed supplemented with 200 mg/kg DM of vitamin E (MR
200). The disappearance of linoleic acid (LA), alpha-linolenic acid (LnA), oleic acid (OA), and appearance of stearic acid (SA), and C18:1 trans-11 (VA) were significantly decreased by micronization (P < 0.0001 for all). The appearance of cis-9, trans-11 conjugated linoleic acid (CLA; P < 0.0001) and C18:1 trans-10 (P = 0.01) was significantly increased by micronization. Biohydrogenation of LA (P = 0.03) and OA (P = 0.02) was significantly decreased by vitamin E. There was a tendency to reduce disappearance of LnA by vitamin E (P = 0.06). In conclusion, micronization is an effective method to protect unsaturated fatty acids (UFA) from ruminal BH. Vitamin E supplementation can be an advantageous strategy to decrease LA, LnA and OA biohydrogenation.
KW - Whole flaked rapeseed
KW - micronization
KW - rumen biohydrogenation
KW - unsaturated fatty acid
KW - vitamin E
UR - http://www.scopus.com/inward/record.url?scp=85180470136&partnerID=8YFLogxK
U2 - 10.1080/09712119.2023.2290124
DO - 10.1080/09712119.2023.2290124
M3 - Journal article
SN - 0971-2119
VL - 52
JO - Journal of Applied Animal Research
JF - Journal of Applied Animal Research
IS - 1
M1 - 2290124
ER -