Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis. / Schlafer, Sebastian; Garcia, Javier; Meyer, Rikke L.; Vaeth, Michael; Neuhaus, Klaus W.

In: European Endodontic Journal, Vol. 3, No. 2, 2018, p. 82-86.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{6648f0ab4c0b4a2bb9f760aa9eb6f83f,
title = "Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis",
abstract = "Objective: Extracellular DNA (eDNA) has been shown to be important for biofilm stability of the endodontic pathogen Enterococcus faecalis. In this study, we hypothesized that treatment with DNase prevents adhesion and disperses young E. faecalis biofilms in 96-well plates and root canals of extracted teeth.Methods: E. faecalis eDNA in 96-well plates was visualized with TOTO-1 (R). The effect of DNase treatment was assessed in 96-well plates and in extracted single-rooted premolars (n=37) using a two-phase crossover design. E. faecalis was treated with DNase (50 Kunitz/mL) or heat-inactivated DNase for 1 h during adhesion or after 24 h of biofilm formation. In 96-well plates, adhering cells were quantified using confocal microscopy and digital image analysis. In root canals, the number of adhering cells was determined in dentine samples based on colony forming unit counts. Data from the 96-well plate were analyzed using one-tailed t-tests, and data from extracted teeth were analyzed using mixed-effect Poisson regressions.Results: eDNA was present in wells colonized by E. faecalis after 1 h of adhesion and 24 h of biofilm formation; it was removed by DNase treatment, as evidenced by TOTO (R)-1 staining. DNase treatment reduced the area covered by cells in 96-well plates after 1 h (pConclusion: DNase treatment does not disperse endodontic E. faecalis biofilms. The sole use of DNase as an anti-biofilm agent in root canal treatments is not recommendable.",
keywords = "Adhesion, biofilm, DNase, Enterococcus faecalis, endodontic biofilms, extracellular DNA, EXTRACELLULAR DNA, EX-VIVO, RELEASE, AUTOLYSIS",
author = "Sebastian Schlafer and Javier Garcia and Meyer, {Rikke L.} and Michael Vaeth and Neuhaus, {Klaus W.}",
year = "2018",
doi = "10.14744/eej.2018.55264",
language = "English",
volume = "3",
pages = "82--86",
journal = "European Endodontic Journal",
issn = "2548-0839",
publisher = "AVES PRESS LTD",
number = "2",

}

RIS

TY - JOUR

T1 - Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis

AU - Schlafer, Sebastian

AU - Garcia, Javier

AU - Meyer, Rikke L.

AU - Vaeth, Michael

AU - Neuhaus, Klaus W.

PY - 2018

Y1 - 2018

N2 - Objective: Extracellular DNA (eDNA) has been shown to be important for biofilm stability of the endodontic pathogen Enterococcus faecalis. In this study, we hypothesized that treatment with DNase prevents adhesion and disperses young E. faecalis biofilms in 96-well plates and root canals of extracted teeth.Methods: E. faecalis eDNA in 96-well plates was visualized with TOTO-1 (R). The effect of DNase treatment was assessed in 96-well plates and in extracted single-rooted premolars (n=37) using a two-phase crossover design. E. faecalis was treated with DNase (50 Kunitz/mL) or heat-inactivated DNase for 1 h during adhesion or after 24 h of biofilm formation. In 96-well plates, adhering cells were quantified using confocal microscopy and digital image analysis. In root canals, the number of adhering cells was determined in dentine samples based on colony forming unit counts. Data from the 96-well plate were analyzed using one-tailed t-tests, and data from extracted teeth were analyzed using mixed-effect Poisson regressions.Results: eDNA was present in wells colonized by E. faecalis after 1 h of adhesion and 24 h of biofilm formation; it was removed by DNase treatment, as evidenced by TOTO (R)-1 staining. DNase treatment reduced the area covered by cells in 96-well plates after 1 h (pConclusion: DNase treatment does not disperse endodontic E. faecalis biofilms. The sole use of DNase as an anti-biofilm agent in root canal treatments is not recommendable.

AB - Objective: Extracellular DNA (eDNA) has been shown to be important for biofilm stability of the endodontic pathogen Enterococcus faecalis. In this study, we hypothesized that treatment with DNase prevents adhesion and disperses young E. faecalis biofilms in 96-well plates and root canals of extracted teeth.Methods: E. faecalis eDNA in 96-well plates was visualized with TOTO-1 (R). The effect of DNase treatment was assessed in 96-well plates and in extracted single-rooted premolars (n=37) using a two-phase crossover design. E. faecalis was treated with DNase (50 Kunitz/mL) or heat-inactivated DNase for 1 h during adhesion or after 24 h of biofilm formation. In 96-well plates, adhering cells were quantified using confocal microscopy and digital image analysis. In root canals, the number of adhering cells was determined in dentine samples based on colony forming unit counts. Data from the 96-well plate were analyzed using one-tailed t-tests, and data from extracted teeth were analyzed using mixed-effect Poisson regressions.Results: eDNA was present in wells colonized by E. faecalis after 1 h of adhesion and 24 h of biofilm formation; it was removed by DNase treatment, as evidenced by TOTO (R)-1 staining. DNase treatment reduced the area covered by cells in 96-well plates after 1 h (pConclusion: DNase treatment does not disperse endodontic E. faecalis biofilms. The sole use of DNase as an anti-biofilm agent in root canal treatments is not recommendable.

KW - Adhesion

KW - biofilm

KW - DNase

KW - Enterococcus faecalis

KW - endodontic biofilms

KW - extracellular DNA

KW - EXTRACELLULAR DNA

KW - EX-VIVO

KW - RELEASE

KW - AUTOLYSIS

U2 - 10.14744/eej.2018.55264

DO - 10.14744/eej.2018.55264

M3 - Journal article

VL - 3

SP - 82

EP - 86

JO - European Endodontic Journal

JF - European Endodontic Journal

SN - 2548-0839

IS - 2

ER -