Effect of DNase treatment on adhesion and early biofilm formation of Enterococcus faecalis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

Objective: Extracellular DNA (eDNA) has been shown to be important for biofilm stability of the endodontic pathogen Enterococcus faecalis. In this study, we hypothesized that treatment with DNase prevents adhesion and disperses young E. faecalis biofilms in 96-well plates and root canals of extracted teeth.

Methods: E. faecalis eDNA in 96-well plates was visualized with TOTO-1 (R). The effect of DNase treatment was assessed in 96-well plates and in extracted single-rooted premolars (n=37) using a two-phase crossover design. E. faecalis was treated with DNase (50 Kunitz/mL) or heat-inactivated DNase for 1 h during adhesion or after 24 h of biofilm formation. In 96-well plates, adhering cells were quantified using confocal microscopy and digital image analysis. In root canals, the number of adhering cells was determined in dentine samples based on colony forming unit counts. Data from the 96-well plate were analyzed using one-tailed t-tests, and data from extracted teeth were analyzed using mixed-effect Poisson regressions.

Results: eDNA was present in wells colonized by E. faecalis after 1 h of adhesion and 24 h of biofilm formation; it was removed by DNase treatment, as evidenced by TOTO (R)-1 staining. DNase treatment reduced the area covered by cells in 96-well plates after 1 h (p

Conclusion: DNase treatment does not disperse endodontic E. faecalis biofilms. The sole use of DNase as an anti-biofilm agent in root canal treatments is not recommendable.

Original languageEnglish
JournalEuropean Endodontic Journal
Volume3
Issue2
Pages (from-to)82-86
Number of pages5
ISSN2548-0839
DOIs
Publication statusPublished - 2018

    Research areas

  • Adhesion, biofilm, DNase, Enterococcus faecalis, endodontic biofilms, extracellular DNA, EXTRACELLULAR DNA, EX-VIVO, RELEASE, AUTOLYSIS

See relations at Aarhus University Citationformats

ID: 132238339