TY - JOUR
T1 - Early effects of eccentric contractions on muscle glucose uptake
AU - Andersen, Ole Emil
AU - Nielsen, Ole Bækgaard
AU - Overgaard, Kristian
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Muscle-damaging eccentric exercise impairs muscle glucose uptake several hours to days after exercise. Little, however, is known about the acute effects of eccentric exercise on contraction- and insulin-induced glucose uptake. This study compares glucose uptake rates in the first hours following eccentric, concentric, and isometric contractions with and without insulin present. Isolated rat extensor digitorum longus muscles were exposed to either an eccentric, concentric, or isometric contraction protocol, and muscle contractions were induced by electric stimulation that was identical between contraction protocols. In eccentric and concentric modes, length changes of 0.6 or 1.2 mm were used during contractions. Both contraction- and insulin-induced glucose uptake were assessed immediately and 2 h after contractions. Glucose uptake increased significantly following all modes of contraction and was higher after eccentric contractions with a stretch of 1.2 mm compared with the remaining contraction groups when assessed immediately after contractions [eccentric (1.2 mm) eccentric (0.6 mm), concentric (1.2 mm), concentric (0.6 mm), isometric rest; P 0.05]. After 2 h, contraction-induced glucose uptake was still higher than noncontracting levels, but with no difference between contraction modes. The presence of insulin increased glucose uptake markedly, but this response was blunted by, respectively, 39 –51% and 29 –36% (P 0.05) immediately and 2 h after eccentric contractions stretched 1.2 mm compared with concentric and isometric contractions. The contrasting early effects of eccentric contractions on contraction- and insulin-induced glucose uptake suggest that glucose uptake is impaired acutely following eccentric exercise because of reduced insulin responsiveness.
AB - Muscle-damaging eccentric exercise impairs muscle glucose uptake several hours to days after exercise. Little, however, is known about the acute effects of eccentric exercise on contraction- and insulin-induced glucose uptake. This study compares glucose uptake rates in the first hours following eccentric, concentric, and isometric contractions with and without insulin present. Isolated rat extensor digitorum longus muscles were exposed to either an eccentric, concentric, or isometric contraction protocol, and muscle contractions were induced by electric stimulation that was identical between contraction protocols. In eccentric and concentric modes, length changes of 0.6 or 1.2 mm were used during contractions. Both contraction- and insulin-induced glucose uptake were assessed immediately and 2 h after contractions. Glucose uptake increased significantly following all modes of contraction and was higher after eccentric contractions with a stretch of 1.2 mm compared with the remaining contraction groups when assessed immediately after contractions [eccentric (1.2 mm) eccentric (0.6 mm), concentric (1.2 mm), concentric (0.6 mm), isometric rest; P 0.05]. After 2 h, contraction-induced glucose uptake was still higher than noncontracting levels, but with no difference between contraction modes. The presence of insulin increased glucose uptake markedly, but this response was blunted by, respectively, 39 –51% and 29 –36% (P 0.05) immediately and 2 h after eccentric contractions stretched 1.2 mm compared with concentric and isometric contractions. The contrasting early effects of eccentric contractions on contraction- and insulin-induced glucose uptake suggest that glucose uptake is impaired acutely following eccentric exercise because of reduced insulin responsiveness.
KW - eccentric
KW - glucose uptake
KW - glycogen
KW - insulin
KW - recovery
UR - http://www.scopus.com/inward/record.url?scp=85061619049&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.00388.2018
DO - 10.1152/japplphysiol.00388.2018
M3 - Journal article
C2 - 30543500
AN - SCOPUS:85061619049
SN - 8750-7587
VL - 126
SP - 376
EP - 385
JO - Journal of applied physiology (Bethesda, Md. : 1985)
JF - Journal of applied physiology (Bethesda, Md. : 1985)
IS - 2
ER -