Dramatically improved RNA in situ hybridization signals using LNA-modified probes.

TY - JOUR

T1 - Dramatically improved RNA in situ hybridization signals using LNA-modified probes.

AU - Thomsen, Rune

AU - Nielsen, Peter Stein

AU - Jensen, Torben Heick

PY - 2005/9/21

Y1 - 2005/9/21

N2 - In situ detection of RNA by hybridization with complementary probes is a powerful technique. Probe design is a critical parameter in successful target detection. We have evaluated the efficiency of fluorescent DNA oligonucleotides modified to contain locked nucleic acid (LNA) residues. This increases the thermal stability of hybrids formed with RNA. The LNA-based probes detect specific RNAs in fixed yeast cells with an efficiency far better than conventional DNA oligonucleotide probes of the same sequence. Using this probe design, we were also able to detect poly(A)+ RNA accumulation within the nucleus/ nucleolus of wild-type cells. LNA-based probes should be readily applicable to a diverse array of cells and tissue samples.

AB - In situ detection of RNA by hybridization with complementary probes is a powerful technique. Probe design is a critical parameter in successful target detection. We have evaluated the efficiency of fluorescent DNA oligonucleotides modified to contain locked nucleic acid (LNA) residues. This increases the thermal stability of hybrids formed with RNA. The LNA-based probes detect specific RNAs in fixed yeast cells with an efficiency far better than conventional DNA oligonucleotide probes of the same sequence. Using this probe design, we were also able to detect poly(A)+ RNA accumulation within the nucleus/ nucleolus of wild-type cells. LNA-based probes should be readily applicable to a diverse array of cells and tissue samples.

U2 - 10.1261/rna.2139705

DO - 10.1261/rna.2139705

M3 - Journal article

C2 - 16177135

SN - 1355-8382

VL - 11

SP - 1745

EP - 1748

JO - RNA

JF - RNA

ER -