Docking of Antibodies into Cavities in DNA Origami

X Quyang; Mattia De Stefano; Abhichart Krissanaprasit; Anne Louise Bank Kodal; Christian Bech Rosen; T Liu; Sarah Wendelbo Helmig; C Fan; Kurt Vesterager Gothelf

doi:10.1002/anie.201706765

Docking of Antibodies into Cavities in DNA Origami

X Quyang , Mattia De Stefano, Abhichart Krissanaprasit, Anne Louise Bank Kodal, Christian Bech Rosen, T Liu, Sarah Wendelbo Helmig, C Fan, Kurt Vesterager Gothelf

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

54 Citations (Scopus)

Abstract

Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.

Original language	English
Journal	Angewandte Chemie International Edition
Volume	56
Issue	46
Pages (from-to)	14423-14427
Number of pages	5
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201706765
Publication status	Published - 13 Dec 2017

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10.1002/anie.201706765

Cite this

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title = "Docking of Antibodies into Cavities in DNA Origami",

abstract = "Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.",

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T1 - Docking of Antibodies into Cavities in DNA Origami

AU - Quyang , X

AU - Stefano, Mattia De

AU - Krissanaprasit, Abhichart

AU - Kodal, Anne Louise Bank

AU - Rosen, Christian Bech

AU - Liu, T

AU - Helmig, Sarah Wendelbo

AU - Fan, C

AU - Gothelf, Kurt Vesterager

PY - 2017/12/13

Y1 - 2017/12/13

N2 - Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.

AB - Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.

U2 - 10.1002/anie.201706765

DO - 10.1002/anie.201706765

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