DNA-Templated Introduction of an Aldehyde Handle in Proteins

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

DNA-Templated Introduction of an Aldehyde Handle in Proteins. / Kodal, Anne Louise Bank; Rosen, Christian Bech; Mortensen, Michael Rosholm; Tørring, Thomas; Gothelf, Kurt Vesterager.

In: ChemBioChem, Vol. 17, No. 14, 2016, p. 1338-1342.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Kodal, ALB, Rosen, CB, Mortensen, MR, Tørring, T & Gothelf, KV 2016, 'DNA-Templated Introduction of an Aldehyde Handle in Proteins', ChemBioChem, vol. 17, no. 14, pp. 1338-1342. https://doi.org/10.1002/cbic.201600254

APA

Kodal, A. L. B., Rosen, C. B., Mortensen, M. R., Tørring, T., & Gothelf, K. V. (2016). DNA-Templated Introduction of an Aldehyde Handle in Proteins. ChemBioChem, 17(14), 1338-1342. https://doi.org/10.1002/cbic.201600254

CBE

MLA

Kodal, Anne Louise Bank et al. "DNA-Templated Introduction of an Aldehyde Handle in Proteins". ChemBioChem. 2016, 17(14). 1338-1342. https://doi.org/10.1002/cbic.201600254

Vancouver

Author

Kodal, Anne Louise Bank ; Rosen, Christian Bech ; Mortensen, Michael Rosholm ; Tørring, Thomas ; Gothelf, Kurt Vesterager. / DNA-Templated Introduction of an Aldehyde Handle in Proteins. In: ChemBioChem. 2016 ; Vol. 17, No. 14. pp. 1338-1342.

Bibtex

@article{8d65cd95c26540a4bb77d33cb9d7d7c3,
title = "DNA-Templated Introduction of an Aldehyde Handle in Proteins",
abstract = "Many medical and biotechnological applications rely on labeling of proteins, but one key challenge is the production of homogeneous and site-specific conjugates. This can rarely be achieved by mere residue-specific random labeling, but requires genetic engineering. Using site-selective DNA-templated reductive amination we create DNA-protein conjugates with control over labeling stoichiometry without genetic engineering. A guiding DNA strand with a metal-binding functionality facilitates site-selectivity by directing coupling of a second reactive DNA strand to the vicinity of a protein metal-binding site. Here, we demonstrate DNA-templated reductive amination for His6-tagged proteins and native metal-binding proteins, including IgG1 antibodies. We also use a cleavable linker between the DNA and the protein to remove the DNA and introduce a single aldehyde to proteins. This functions as a handle for further modifications with desired labels. In addition to directing the aldehyde positioning, the DNA provides a straightforward route of purification between reaction steps.",
author = "Kodal, {Anne Louise Bank} and Rosen, {Christian Bech} and Mortensen, {Michael Rosholm} and Thomas T{\o}rring and Gothelf, {Kurt Vesterager}",
year = "2016",
doi = "10.1002/cbic.201600254",
language = "English",
volume = "17",
pages = "1338--1342",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "14",

}

RIS

TY - JOUR

T1 - DNA-Templated Introduction of an Aldehyde Handle in Proteins

AU - Kodal, Anne Louise Bank

AU - Rosen, Christian Bech

AU - Mortensen, Michael Rosholm

AU - Tørring, Thomas

AU - Gothelf, Kurt Vesterager

PY - 2016

Y1 - 2016

N2 - Many medical and biotechnological applications rely on labeling of proteins, but one key challenge is the production of homogeneous and site-specific conjugates. This can rarely be achieved by mere residue-specific random labeling, but requires genetic engineering. Using site-selective DNA-templated reductive amination we create DNA-protein conjugates with control over labeling stoichiometry without genetic engineering. A guiding DNA strand with a metal-binding functionality facilitates site-selectivity by directing coupling of a second reactive DNA strand to the vicinity of a protein metal-binding site. Here, we demonstrate DNA-templated reductive amination for His6-tagged proteins and native metal-binding proteins, including IgG1 antibodies. We also use a cleavable linker between the DNA and the protein to remove the DNA and introduce a single aldehyde to proteins. This functions as a handle for further modifications with desired labels. In addition to directing the aldehyde positioning, the DNA provides a straightforward route of purification between reaction steps.

AB - Many medical and biotechnological applications rely on labeling of proteins, but one key challenge is the production of homogeneous and site-specific conjugates. This can rarely be achieved by mere residue-specific random labeling, but requires genetic engineering. Using site-selective DNA-templated reductive amination we create DNA-protein conjugates with control over labeling stoichiometry without genetic engineering. A guiding DNA strand with a metal-binding functionality facilitates site-selectivity by directing coupling of a second reactive DNA strand to the vicinity of a protein metal-binding site. Here, we demonstrate DNA-templated reductive amination for His6-tagged proteins and native metal-binding proteins, including IgG1 antibodies. We also use a cleavable linker between the DNA and the protein to remove the DNA and introduce a single aldehyde to proteins. This functions as a handle for further modifications with desired labels. In addition to directing the aldehyde positioning, the DNA provides a straightforward route of purification between reaction steps.

U2 - 10.1002/cbic.201600254

DO - 10.1002/cbic.201600254

M3 - Journal article

VL - 17

SP - 1338

EP - 1342

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 14

ER -