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DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

Research output: Contribution to book/anthology/report/proceedingArticle in proceedingsResearchpeer-review

  • V. Ferrara, Università di Catania
  • ,
  • A. Ottaviani, Universita degli Studi di Roma Tor Vergata
  • ,
  • F. Cavaleri, Universita degli Studi di Palermo
  • ,
  • G. Arrabito, Universita degli Studi di Palermo
  • ,
  • P. Cancemi, Universita degli Studi di Palermo
  • ,
  • Y. P. Ho, Chinese University Hong Kong
  • ,
  • B. R. Knudsen
  • M. S. Hede
  • C. Pellerito, Universita degli Studi di Palermo
  • ,
  • A. Desideri, Universita degli Studi di Roma Tor Vergata
  • ,
  • S. Feo, Universita degli Studi di Palermo
  • ,
  • Giovanni Marletta, Università di Catania
  • ,
  • B. Pignataro, Universita degli Studi di Palermo

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

Original languageEnglish
Title of host publicationSensors
EditorsBruno Andò, Francesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Vincenzo Marletta, Giovanna Marrazza
Number of pages8
PublisherSpringer
Publication year18 Jan 2019
Pages309-316
ISBN (print)9783030043230
ISBN (Electronic)978-3-030-04324-7
DOIs
Publication statusPublished - 18 Jan 2019
Event4th National Conference on Sensors, 2018 - Catania, Italy
Duration: 21 Feb 201823 Feb 2018

Conference

Conference4th National Conference on Sensors, 2018
LandItaly
ByCatania
Periode21/02/201823/02/2018
SeriesLecture Notes in Electrical Engineering
Volume539
ISSN1876-1100

    Research areas

  • Biosensor, Flexible device, Molecular printing, Topoisomerase

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