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DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection
Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review
DOI
- https://doi.org/10.1007/978-3-030-04324-7_39
Final published version
- V. Ferrara, Università di Catania ,
- A. Ottaviani, Universita degli Studi di Roma Tor Vergata ,
- F. Cavaleri, Universita degli Studi di Palermo ,
- G. Arrabito, Universita degli Studi di Palermo ,
- P. Cancemi, Universita degli Studi di Palermo ,
- Y. P. Ho, Chinese University Hong Kong ,
- B. R. Knudsen
- M. S. Hede
- C. Pellerito, Universita degli Studi di Palermo ,
- A. Desideri, Universita degli Studi di Roma Tor Vergata ,
- S. Feo, Universita degli Studi di Palermo ,
- Giovanni Marletta, Università di Catania ,
- B. Pignataro, Universita degli Studi di Palermo
Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.
| Original language | English |
|---|---|
| Title of host publication | Sensors |
| Editors | Bruno Andò, Francesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Vincenzo Marletta, Giovanna Marrazza |
| Number of pages | 8 |
| Publisher | Springer |
| Publication year | 18 Jan 2019 |
| Pages | 309-316 |
| ISBN (print) | 9783030043230 |
| ISBN (Electronic) | 978-3-030-04324-7 |
| DOIs | |
| Publication status | Published - 18 Jan 2019 |
| Event | 4th National Conference on Sensors, 2018 - Catania, Italy Duration: 21 Feb 2018 → 23 Feb 2018 |
Conference
| Conference | 4th National Conference on Sensors, 2018 |
|---|---|
| Land | Italy |
| By | Catania |
| Periode | 21/02/2018 → 23/02/2018 |
| Series | Lecture Notes in Electrical Engineering |
|---|---|
| Volume | 539 |
| ISSN | 1876-1100 |
- Biosensor, Flexible device, Molecular printing, Topoisomerase
Research areas
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