DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

Morten Roedgaard; Jacob Fredsoe; Jakob Madsen Pedersen; Lotte Bjergbaek; Anni Hangaard Andersen

doi:10.1371/journal.pone.0132739

DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

Morten Roedgaard, Jacob Fredsoe, Jakob Madsen Pedersen, Lotte Bjergbaek, Anni Hangaard Andersen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.

Original language	English
Article number	e0132739
Journal	PLOS ONE
Volume	10
Issue	7
Pages (from-to)	1-15
Number of pages	15
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0132739
Publication status	Published - 14 Jul 2015

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title = "DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation",

abstract = "To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.",

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DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation. / Roedgaard, Morten; Fredsoe, Jacob; Pedersen, Jakob Madsen et al.
In: PLOS ONE, Vol. 10, No. 7, e0132739, 14.07.2015, p. 1-15.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

AU - Roedgaard, Morten

AU - Fredsoe, Jacob

AU - Pedersen, Jakob Madsen

AU - Bjergbaek, Lotte

AU - Andersen, Anni Hangaard

PY - 2015/7/14

Y1 - 2015/7/14

N2 - To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.

AB - To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.

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DO - 10.1371/journal.pone.0132739

M3 - Journal article

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EP - 15

JO - PLOS ONE

JF - PLOS ONE

IS - 7

M1 - e0132739

ER -

DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

Abstract

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