DNA origami design of 3D nanostructures

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DNA origami design of 3D nanostructures. / Andersen, Ebbe Sloth; Nielsen, Morten Muhlig.

In: Nature Protocols (Online), 2009.

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@article{318b1230bf7911df8cb9000ea68e967b,
title = "DNA origami design of 3D nanostructures",
abstract = "Structural DNA nanotechnology has been heavily dependent on the development of dedicated software tools for the design of unique helical junctions, to define unique sticky-ends for tile assembly, and for predicting the products of the self-assembly reaction of multiple DNA strands [1-3]. Recently, several dedicated 3D editors for computer-aided design of DNA structures have been developed [4-7]. However, many of these tools are not efficient for designing DNA origami structures that requires the design of more than 200 unique DNA strands to be folded along a scaffold strand into a defined 3D shape [8]. We have recently developed a semi-automated DNA origami software package [9] that uses a 2D sequence editor in conjunction with several automated tools to facilitate the design process. Here we extend the use of the program for designing DNA origami structures in 3D and show the application by the construction of a DNA box with dimensions of 42 × 36 × 36 nm3. The software is available at www.cdna.dk/origami/ .",
author = "Andersen, {Ebbe Sloth} and Nielsen, {Morten Muhlig}",
note = "Paper id:: 10.1038/nprot.2009.75",
year = "2009",
language = "English",
journal = "Nature Protocols (Online)",
issn = "1750-2799",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - DNA origami design of 3D nanostructures

AU - Andersen, Ebbe Sloth

AU - Nielsen, Morten Muhlig

N1 - Paper id:: 10.1038/nprot.2009.75

PY - 2009

Y1 - 2009

N2 - Structural DNA nanotechnology has been heavily dependent on the development of dedicated software tools for the design of unique helical junctions, to define unique sticky-ends for tile assembly, and for predicting the products of the self-assembly reaction of multiple DNA strands [1-3]. Recently, several dedicated 3D editors for computer-aided design of DNA structures have been developed [4-7]. However, many of these tools are not efficient for designing DNA origami structures that requires the design of more than 200 unique DNA strands to be folded along a scaffold strand into a defined 3D shape [8]. We have recently developed a semi-automated DNA origami software package [9] that uses a 2D sequence editor in conjunction with several automated tools to facilitate the design process. Here we extend the use of the program for designing DNA origami structures in 3D and show the application by the construction of a DNA box with dimensions of 42 × 36 × 36 nm3. The software is available at www.cdna.dk/origami/ .

AB - Structural DNA nanotechnology has been heavily dependent on the development of dedicated software tools for the design of unique helical junctions, to define unique sticky-ends for tile assembly, and for predicting the products of the self-assembly reaction of multiple DNA strands [1-3]. Recently, several dedicated 3D editors for computer-aided design of DNA structures have been developed [4-7]. However, many of these tools are not efficient for designing DNA origami structures that requires the design of more than 200 unique DNA strands to be folded along a scaffold strand into a defined 3D shape [8]. We have recently developed a semi-automated DNA origami software package [9] that uses a 2D sequence editor in conjunction with several automated tools to facilitate the design process. Here we extend the use of the program for designing DNA origami structures in 3D and show the application by the construction of a DNA box with dimensions of 42 × 36 × 36 nm3. The software is available at www.cdna.dk/origami/ .

M3 - Comment/debate

JO - Nature Protocols (Online)

JF - Nature Protocols (Online)

SN - 1750-2799

ER -