DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

V. Ferrara; A. Ottaviani; F. Cavaleri; G. Arrabito; P. Cancemi; Y. P. Ho; B. R. Knudsen; M. S. Hede; C. Pellerito; A. Desideri; S. Feo; Giovanni Marletta; B. Pignataro

doi:10.1007/978-3-030-04324-7_39

DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

V. Ferrara, A. Ottaviani, F. Cavaleri, G. Arrabito, P. Cancemi, Y. P. Ho, B. R. Knudsen, M. S. Hede, C. Pellerito, A. Desideri, S. Feo, Giovanni Marletta, B. Pignataro^*

^*Corresponding author for this work

Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review

1 Citation (Scopus)

Abstract

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

Original language	English
Title of host publication	Sensors
Editors	Bruno Andò, Francesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Vincenzo Marletta, Giovanna Marrazza
Number of pages	8
Publisher	Springer
Publication date	18 Jan 2019
Pages	309-316
ISBN (Print)	9783030043230
ISBN (Electronic)	978-3-030-04324-7
DOIs	https://doi.org/10.1007/978-3-030-04324-7_39
Publication status	Published - 18 Jan 2019
Event	4th National Conference on Sensors, 2018 - Catania, Italy Duration: 21 Feb 2018 → 23 Feb 2018

Conference

Conference	4th National Conference on Sensors, 2018
Country/Territory	Italy
City	Catania
Period	21/02/2018 → 23/02/2018

Series	Lecture Notes in Electrical Engineering
Volume	539
ISSN	1876-1100

Keywords

Biosensor
Flexible device
Molecular printing
Topoisomerase

Access to Document

10.1007/978-3-030-04324-7_39

Cite this

Ferrara, V., Ottaviani, A., Cavaleri, F., Arrabito, G., Cancemi, P., Ho, Y. P., Knudsen, B. R., Hede, M. S., Pellerito, C., Desideri, A., Feo, S., Marletta, G., & Pignataro, B. (2019). DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. In B. Andò, F. Baldini, P. Siciliano, M. Rossi, L. Scalise, C. Di Natale, V. Ferrari, V. Militello, G. Miolo, V. Marletta, & G. Marrazza (Eds.), Sensors (pp. 309-316). Springer. https://doi.org/10.1007/978-3-030-04324-7_39

Ferrara, V. ; Ottaviani, A. ; Cavaleri, F. et al. / DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. Sensors. editor / Bruno Andò ; Francesco Baldini ; Pietro Siciliano ; Marco Rossi ; Lorenzo Scalise ; Corrado Di Natale ; Vittorio Ferrari ; Valeria Militello ; Giorgia Miolo ; Vincenzo Marletta ; Giovanna Marrazza. Springer, 2019. pp. 309-316 (Lecture Notes in Electrical Engineering, Vol. 539).

@inproceedings{3d2c56c302f349e59f6cb0151f9d9e81,

title = "DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection",

abstract = "Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.",

keywords = "Biosensor, Flexible device, Molecular printing, Topoisomerase",

author = "V. Ferrara and A. Ottaviani and F. Cavaleri and G. Arrabito and P. Cancemi and Ho, {Y. P.} and Knudsen, {B. R.} and Hede, {M. S.} and C. Pellerito and A. Desideri and S. Feo and Giovanni Marletta and B. Pignataro",

year = "2019",

month = jan,

day = "18",

doi = "10.1007/978-3-030-04324-7_39",

language = "English",

isbn = "9783030043230",

series = "Lecture Notes in Electrical Engineering",

publisher = "Springer",

pages = "309--316",

editor = "Bruno And{\`o} and Francesco Baldini and Pietro Siciliano and Marco Rossi and Lorenzo Scalise and {Di Natale}, Corrado and Vittorio Ferrari and Valeria Militello and Giorgia Miolo and Vincenzo Marletta and Giovanna Marrazza",

booktitle = "Sensors",

address = "Netherlands",

note = "4th National Conference on Sensors, 2018 ; Conference date: 21-02-2018 Through 23-02-2018",

}

Ferrara, V, Ottaviani, A, Cavaleri, F, Arrabito, G, Cancemi, P, Ho, YP, Knudsen, BR, Hede, MS, Pellerito, C, Desideri, A, Feo, S, Marletta, G & Pignataro, B 2019, DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. in B Andò, F Baldini, P Siciliano, M Rossi, L Scalise, C Di Natale, V Ferrari, V Militello, G Miolo, V Marletta & G Marrazza (eds), Sensors. Springer, Lecture Notes in Electrical Engineering, vol. 539, pp. 309-316, 4th National Conference on Sensors, 2018, Catania, Italy, 21/02/2018. https://doi.org/10.1007/978-3-030-04324-7_39

DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. / Ferrara, V.; Ottaviani, A.; Cavaleri, F. et al.
Sensors. ed. / Bruno Andò; Francesco Baldini; Pietro Siciliano; Marco Rossi; Lorenzo Scalise; Corrado Di Natale; Vittorio Ferrari; Valeria Militello; Giorgia Miolo; Vincenzo Marletta; Giovanna Marrazza. Springer, 2019. p. 309-316 (Lecture Notes in Electrical Engineering, Vol. 539).

Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

AU - Ferrara, V.

AU - Ottaviani, A.

AU - Cavaleri, F.

AU - Arrabito, G.

AU - Cancemi, P.

AU - Ho, Y. P.

AU - Knudsen, B. R.

AU - Hede, M. S.

AU - Pellerito, C.

AU - Desideri, A.

AU - Feo, S.

AU - Marletta, Giovanni

AU - Pignataro, B.

PY - 2019/1/18

Y1 - 2019/1/18

N2 - Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

AB - Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

KW - Biosensor

KW - Flexible device

KW - Molecular printing

KW - Topoisomerase

UR - http://www.scopus.com/inward/record.url?scp=85061082536&partnerID=8YFLogxK

U2 - 10.1007/978-3-030-04324-7_39

DO - 10.1007/978-3-030-04324-7_39

M3 - Article in proceedings

AN - SCOPUS:85061082536

SN - 9783030043230

T3 - Lecture Notes in Electrical Engineering

SP - 309

EP - 316

BT - Sensors

A2 - Andò, Bruno

A2 - Baldini, Francesco

A2 - Siciliano, Pietro

A2 - Rossi, Marco

A2 - Scalise, Lorenzo

A2 - Di Natale, Corrado

A2 - Ferrari, Vittorio

A2 - Militello, Valeria

A2 - Miolo, Giorgia

A2 - Marletta, Vincenzo

A2 - Marrazza, Giovanna

PB - Springer

T2 - 4th National Conference on Sensors, 2018

Y2 - 21 February 2018 through 23 February 2018

ER -

Ferrara V, Ottaviani A, Cavaleri F, Arrabito G, Cancemi P, Ho YP et al. DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. In Andò B, Baldini F, Siciliano P, Rossi M, Scalise L, Di Natale C, Ferrari V, Militello V, Miolo G, Marletta V, Marrazza G, editors, Sensors. Springer. 2019. p. 309-316. (Lecture Notes in Electrical Engineering, Vol. 539). doi: 10.1007/978-3-030-04324-7_39

DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this