Disulphide-mediated site-directed modification of proteins

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Standard

Disulphide-mediated site-directed modification of proteins. / Nielsen, Thorbjørn; Märcher, Anders; Drobňáková, Zuzana; Hučko, Michal; Štengl, Milan; Balšánek, Vojtěch; Wiberg, Charlotte; Nielsen, Per F.; Nielsen, Thomas E.; Gothelf, Kurt V.; Cló, Emiliano.

In: Organic and Biomolecular Chemistry, Vol. 18, No. 25, 07.2020, p. 4717-4722.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Nielsen, T, Märcher, A, Drobňáková, Z, Hučko, M, Štengl, M, Balšánek, V, Wiberg, C, Nielsen, PF, Nielsen, TE, Gothelf, KV & Cló, E 2020, 'Disulphide-mediated site-directed modification of proteins', Organic and Biomolecular Chemistry, vol. 18, no. 25, pp. 4717-4722. https://doi.org/10.1039/d0ob00861c

APA

Nielsen, T., Märcher, A., Drobňáková, Z., Hučko, M., Štengl, M., Balšánek, V., Wiberg, C., Nielsen, P. F., Nielsen, T. E., Gothelf, K. V., & Cló, E. (2020). Disulphide-mediated site-directed modification of proteins. Organic and Biomolecular Chemistry, 18(25), 4717-4722. https://doi.org/10.1039/d0ob00861c

CBE

Nielsen T, Märcher A, Drobňáková Z, Hučko M, Štengl M, Balšánek V, Wiberg C, Nielsen PF, Nielsen TE, Gothelf KV, Cló E. 2020. Disulphide-mediated site-directed modification of proteins. Organic and Biomolecular Chemistry. 18(25):4717-4722. https://doi.org/10.1039/d0ob00861c

MLA

Vancouver

Nielsen T, Märcher A, Drobňáková Z, Hučko M, Štengl M, Balšánek V et al. Disulphide-mediated site-directed modification of proteins. Organic and Biomolecular Chemistry. 2020 Jul;18(25):4717-4722. https://doi.org/10.1039/d0ob00861c

Author

Nielsen, Thorbjørn ; Märcher, Anders ; Drobňáková, Zuzana ; Hučko, Michal ; Štengl, Milan ; Balšánek, Vojtěch ; Wiberg, Charlotte ; Nielsen, Per F. ; Nielsen, Thomas E. ; Gothelf, Kurt V. ; Cló, Emiliano. / Disulphide-mediated site-directed modification of proteins. In: Organic and Biomolecular Chemistry. 2020 ; Vol. 18, No. 25. pp. 4717-4722.

Bibtex

@article{fd0ae5067b584c53a66c17e7df3310c1,
title = "Disulphide-mediated site-directed modification of proteins",
abstract = "Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is ",
author = "Thorbj{\o}rn Nielsen and Anders M{\"a}rcher and Zuzana Drob{\v n}{\'a}kov{\'a} and Michal Hu{\v c}ko and Milan {\v S}tengl and Vojt{\v e}ch Bal{\v s}{\'a}nek and Charlotte Wiberg and Nielsen, {Per F.} and Nielsen, {Thomas E.} and Gothelf, {Kurt V.} and Emiliano Cl{\'o}",
year = "2020",
month = jul,
doi = "10.1039/d0ob00861c",
language = "English",
volume = "18",
pages = "4717--4722",
journal = "Organic & Biomolecular Chemistry",
issn = "1477-0520",
publisher = "ROYAL SOC CHEMISTRY",
number = "25",

}

RIS

TY - JOUR

T1 - Disulphide-mediated site-directed modification of proteins

AU - Nielsen, Thorbjørn

AU - Märcher, Anders

AU - Drobňáková, Zuzana

AU - Hučko, Michal

AU - Štengl, Milan

AU - Balšánek, Vojtěch

AU - Wiberg, Charlotte

AU - Nielsen, Per F.

AU - Nielsen, Thomas E.

AU - Gothelf, Kurt V.

AU - Cló, Emiliano

PY - 2020/7

Y1 - 2020/7

N2 - Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is

AB - Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is

UR - http://www.scopus.com/inward/record.url?scp=85087468923&partnerID=8YFLogxK

U2 - 10.1039/d0ob00861c

DO - 10.1039/d0ob00861c

M3 - Journal article

C2 - 32525502

AN - SCOPUS:85087468923

VL - 18

SP - 4717

EP - 4722

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1477-0520

IS - 25

ER -