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Disulphide-mediated site-directed modification of proteins

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  • Thorbjørn Nielsen, Novo Nordisk A/S
  • ,
  • Anders Märcher
  • Zuzana Drobňáková, APIGENEX S.r.o.
  • ,
  • Michal Hučko, APIGENEX S.r.o.
  • ,
  • Milan Štengl, APIGENEX S.r.o.
  • ,
  • Vojtěch Balšánek, APIGENEX S.r.o.
  • ,
  • Charlotte Wiberg, Novo Nordisk A/S
  • ,
  • Per F. Nielsen, Novo Nordisk A/S
  • ,
  • Thomas E. Nielsen, Novo Nordisk A/S
  • ,
  • Kurt V. Gothelf
  • Emiliano Cló, Novo Nordisk A/S

Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is

Original languageEnglish
JournalOrganic and Biomolecular Chemistry
Pages (from-to)4717-4722
Number of pages6
Publication statusPublished - Jul 2020

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