Disulphide-mediated site-directed modification of proteins

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Thorbjørn Nielsen
  • Anders Märcher
  • Zuzana Drobňáková, APIGENEX S.r.o.
  • ,
  • Michal Hučko, APIGENEX S.r.o.
  • ,
  • Milan Štengl, APIGENEX S.r.o.
  • ,
  • Vojtěch Balšánek, APIGENEX S.r.o.
  • ,
  • Charlotte Wiberg, Novo Nordisk A/S
  • ,
  • Per F. Nielsen, Novo Nordisk A/S
  • ,
  • Thomas E. Nielsen, Novo Nordisk A/S
  • ,
  • Kurt V. Gothelf
  • Emiliano Cló, Novo Nordisk A/S

Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is

Original languageEnglish
JournalOrganic and Biomolecular Chemistry
Volume18
Issue25
Pages (from-to)4717-4722
Number of pages6
ISSN1477-0520
DOIs
Publication statusPublished - Jul 2020

See relations at Aarhus University Citationformats

ID: 192495950