Distinctive CD8+ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.

Renée M van der Sluis; Juan L García-Rodríguez; Ian Helstrup Nielsen; Albert Gris-Oliver; Jennifer Becker; Bibiana Costa; M Zeeshan Chaudhry; Marvin Werner; Anders Laustsen; Jesper G Pedersen; Kristine R Gammelgaard; Trine H Mogensen; Ulrich Kalinke; Luka Cicin-Sain; Rasmus O Bak; Lasse S Kristensen; Martin R Jakobsen

doi:10.1016/j.celrep.2025.115413

Distinctive CD8⁺ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.

Renée M van der Sluis^*, Juan L García-Rodríguez, Ian Helstrup Nielsen, Albert Gris-Oliver, Jennifer Becker, Bibiana Costa, M Zeeshan Chaudhry, Marvin Werner, Anders Laustsen, Jesper G Pedersen, Kristine R Gammelgaard, Trine H Mogensen, Ulrich Kalinke, Luka Cicin-Sain, Rasmus O Bak, Lasse S Kristensen, Martin R Jakobsen^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Plasmacytoid dendritic cells (pDCs) play a pivotal role in immune responses, particularly against viral infections. pDCs exhibit diverse functions, including interferon production, cytokine secretion, and antigen presentation. Here, we investigate the antigen cross-presentation capacity of pDCs and their role in CD8 + T cell activation. Utilizing a culturing system with CD8 + T cells and autologous pDCs derived from circulating CD34 + hematopoietic stem and progenitor cells, we demonstrate that pDCs efficiently activate CD8 + T cells via cross-presentation, promoting T cell expansion and cytotoxic activity. The antigen presentation capacity of pDCs is comparable to that of monocyte-derived dendritic cells (moDCs) and myeloid dendritic cells, which are known for their efficient antigen-presentation capacity. Transcriptomic analysis reveals genetic signatures in CD8 + T cells activated by pDCs distinct from moDCs, suggesting different activation mechanisms. These findings underscore the importance of pDCs in antigen presentation and their contribution to CD8 + T cell activation.

Original language	English
Article number	115413
Journal	Cell Reports
Volume	44
Issue	3
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2025.115413
Publication status	Published - 25 Mar 2025

Keywords

CD8 T cell
CP: Immunology
IFNa
antigen presentation
dendritic cell
immunity
inflammation
pDC
plasmacytoid dendritic cell

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van der Sluis, R. M., García-Rodríguez, J. L., Nielsen, I. H., Gris-Oliver, A., Becker, J., Costa, B., Chaudhry, M. Z., Werner, M., Laustsen, A., Pedersen, J. G., Gammelgaard, K. R., Mogensen, T. H., Kalinke, U., Cicin-Sain, L., Bak, R. O., Kristensen, L. S., & Jakobsen, M. R. (2025). Distinctive CD8⁺ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells. Cell Reports, 44(3), Article 115413. https://doi.org/10.1016/j.celrep.2025.115413

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title = "Distinctive CD8+ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.",

abstract = "Plasmacytoid dendritic cells (pDCs) play a pivotal role in immune responses, particularly against viral infections. pDCs exhibit diverse functions, including interferon production, cytokine secretion, and antigen presentation. Here, we investigate the antigen cross-presentation capacity of pDCs and their role in CD8 + T cell activation. Utilizing a culturing system with CD8 + T cells and autologous pDCs derived from circulating CD34 + hematopoietic stem and progenitor cells, we demonstrate that pDCs efficiently activate CD8 + T cells via cross-presentation, promoting T cell expansion and cytotoxic activity. The antigen presentation capacity of pDCs is comparable to that of monocyte-derived dendritic cells (moDCs) and myeloid dendritic cells, which are known for their efficient antigen-presentation capacity. Transcriptomic analysis reveals genetic signatures in CD8 + T cells activated by pDCs distinct from moDCs, suggesting different activation mechanisms. These findings underscore the importance of pDCs in antigen presentation and their contribution to CD8 + T cell activation. ",

keywords = "CD8 T cell, CP: Immunology, IFNa, antigen presentation, dendritic cell, immunity, inflammation, pDC, plasmacytoid dendritic cell",

author = "{van der Sluis}, {Ren{\'e}e M} and Garc{\'i}a-Rodr{\'i}guez, {Juan L} and Nielsen, {Ian Helstrup} and Albert Gris-Oliver and Jennifer Becker and Bibiana Costa and Chaudhry, {M Zeeshan} and Marvin Werner and Anders Laustsen and Pedersen, {Jesper G} and Gammelgaard, {Kristine R} and Mogensen, {Trine H} and Ulrich Kalinke and Luka Cicin-Sain and Bak, {Rasmus O} and Kristensen, {Lasse S} and Jakobsen, {Martin R}",

year = "2025",

month = mar,

day = "25",

doi = "10.1016/j.celrep.2025.115413",

language = "English",

volume = "44",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Elsevier Inc.",

number = "3",

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van der Sluis, RM , García-Rodríguez, JL , Nielsen, IH, Gris-Oliver, A, Becker, J, Costa, B, Chaudhry, MZ, Werner, M, Laustsen, A, Pedersen, JG, Gammelgaard, KR, Mogensen, TH, Kalinke, U, Cicin-Sain, L, Bak, RO , Kristensen, LS & Jakobsen, MR 2025, 'Distinctive CD8⁺ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.', Cell Reports, vol. 44, no. 3, 115413. https://doi.org/10.1016/j.celrep.2025.115413

Distinctive CD8⁺ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells. / van der Sluis, Renée M ; García-Rodríguez, Juan L ; Nielsen, Ian Helstrup et al.
In: Cell Reports, Vol. 44, No. 3, 115413, 25.03.2025.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Distinctive CD8+ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.

AU - van der Sluis, Renée M

AU - García-Rodríguez, Juan L

AU - Nielsen, Ian Helstrup

AU - Gris-Oliver, Albert

AU - Becker, Jennifer

AU - Costa, Bibiana

AU - Chaudhry, M Zeeshan

AU - Werner, Marvin

AU - Laustsen, Anders

AU - Pedersen, Jesper G

AU - Gammelgaard, Kristine R

AU - Mogensen, Trine H

AU - Kalinke, Ulrich

AU - Cicin-Sain, Luka

AU - Bak, Rasmus O

AU - Kristensen, Lasse S

AU - Jakobsen, Martin R

PY - 2025/3/25

Y1 - 2025/3/25

N2 - Plasmacytoid dendritic cells (pDCs) play a pivotal role in immune responses, particularly against viral infections. pDCs exhibit diverse functions, including interferon production, cytokine secretion, and antigen presentation. Here, we investigate the antigen cross-presentation capacity of pDCs and their role in CD8 + T cell activation. Utilizing a culturing system with CD8 + T cells and autologous pDCs derived from circulating CD34 + hematopoietic stem and progenitor cells, we demonstrate that pDCs efficiently activate CD8 + T cells via cross-presentation, promoting T cell expansion and cytotoxic activity. The antigen presentation capacity of pDCs is comparable to that of monocyte-derived dendritic cells (moDCs) and myeloid dendritic cells, which are known for their efficient antigen-presentation capacity. Transcriptomic analysis reveals genetic signatures in CD8 + T cells activated by pDCs distinct from moDCs, suggesting different activation mechanisms. These findings underscore the importance of pDCs in antigen presentation and their contribution to CD8 + T cell activation.

AB - Plasmacytoid dendritic cells (pDCs) play a pivotal role in immune responses, particularly against viral infections. pDCs exhibit diverse functions, including interferon production, cytokine secretion, and antigen presentation. Here, we investigate the antigen cross-presentation capacity of pDCs and their role in CD8 + T cell activation. Utilizing a culturing system with CD8 + T cells and autologous pDCs derived from circulating CD34 + hematopoietic stem and progenitor cells, we demonstrate that pDCs efficiently activate CD8 + T cells via cross-presentation, promoting T cell expansion and cytotoxic activity. The antigen presentation capacity of pDCs is comparable to that of monocyte-derived dendritic cells (moDCs) and myeloid dendritic cells, which are known for their efficient antigen-presentation capacity. Transcriptomic analysis reveals genetic signatures in CD8 + T cells activated by pDCs distinct from moDCs, suggesting different activation mechanisms. These findings underscore the importance of pDCs in antigen presentation and their contribution to CD8 + T cell activation.

KW - CD8 T cell

KW - CP: Immunology

KW - IFNa

KW - antigen presentation

KW - dendritic cell

KW - immunity

KW - inflammation

KW - pDC

KW - plasmacytoid dendritic cell

UR - http://www.scopus.com/inward/record.url?scp=86000635547&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2025.115413

DO - 10.1016/j.celrep.2025.115413

M3 - Journal article

C2 - 40073016

SN - 2211-1247

VL - 44

JO - Cell Reports

JF - Cell Reports

IS - 3

M1 - 115413

ER -

Distinctive CD8⁺ T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.

Abstract

Keywords

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