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Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

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Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth. / Kirkegaard, Kirstine Kjær; Fredsted, Palle Villesen; Jensen, Jacob Malte; Hindkjær, Johnny Juhl; Kølvraa, Steen; Ingerslev, Hans Jakob; Lykke-Hartmann, Karin.

In: Gene, Vol. 571, No. 2, 2015, p. 212-220.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{04a920bcb8d54fa0a6115383fb5849f4,
title = "Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth",
abstract = "Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing",
author = "Kirkegaard, {Kirstine Kj{\ae}r} and Fredsted, {Palle Villesen} and Jensen, {Jacob Malte} and Hindkj{\ae}r, {Johnny Juhl} and Steen K{\o}lvraa and Ingerslev, {Hans Jakob} and Karin Lykke-Hartmann",
year = "2015",
doi = "10.1016/j.gene.2015.06.057",
language = "English",
volume = "571",
pages = "212--220",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

AU - Kirkegaard, Kirstine Kjær

AU - Fredsted, Palle Villesen

AU - Jensen, Jacob Malte

AU - Hindkjær, Johnny Juhl

AU - Kølvraa, Steen

AU - Ingerslev, Hans Jakob

AU - Lykke-Hartmann, Karin

PY - 2015

Y1 - 2015

N2 - Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing

AB - Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing

U2 - 10.1016/j.gene.2015.06.057

DO - 10.1016/j.gene.2015.06.057

M3 - Journal article

C2 - 26117173

VL - 571

SP - 212

EP - 220

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -