Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

Kirstine Kjær Kirkegaard; Palle Villesen Fredsted; Jacob Malte Jensen; Johnny Juhl Hindkjær; Steen Kølvraa; Hans Jakob Ingerslev; Karin Lykke-Hartmann

doi:10.1016/j.gene.2015.06.057

Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

Kirstine Kjær Kirkegaard, Palle Villesen Fredsted, Jacob Malte Jensen, Johnny Juhl Hindkjær, Steen Kølvraa, Hans Jakob Ingerslev, Karin Lykke-Hartmann

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

21 Citations (Scopus)

Abstract

Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing

Original language	English
Journal	Gene
Volume	571
Issue	2
Pages (from-to)	212-220
Number of pages	9
ISSN	0378-1119
DOIs	https://doi.org/10.1016/j.gene.2015.06.057
Publication status	Published - 2015

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10.1016/j.gene.2015.06.057

Dissecting Gene expression Networks
Lykke-Hartmann, K.
20/03/2016
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@article{04a920bcb8d54fa0a6115383fb5849f4,

title = "Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth",

abstract = "Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing",

author = "Kirkegaard, {Kirstine Kj{\ae}r} and Fredsted, {Palle Villesen} and Jensen, {Jacob Malte} and Hindkj{\ae}r, {Johnny Juhl} and Steen K{\o}lvraa and Ingerslev, {Hans Jakob} and Karin Lykke-Hartmann",

year = "2015",

doi = "10.1016/j.gene.2015.06.057",

language = "English",

volume = "571",

pages = "212--220",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2",

}

Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth. / Kirkegaard, Kirstine Kjær ; Fredsted, Palle Villesen; Jensen, Jacob Malte et al.
In: Gene, Vol. 571, No. 2, 2015, p. 212-220.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

AU - Kirkegaard, Kirstine Kjær

AU - Fredsted, Palle Villesen

AU - Jensen, Jacob Malte

AU - Hindkjær, Johnny Juhl

AU - Kølvraa, Steen

AU - Ingerslev, Hans Jakob

AU - Lykke-Hartmann, Karin

PY - 2015

Y1 - 2015

N2 - Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing

AB - Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing

U2 - 10.1016/j.gene.2015.06.057

DO - 10.1016/j.gene.2015.06.057

M3 - Journal article

C2 - 26117173

SN - 0378-1119

VL - 571

SP - 212

EP - 220

JO - Gene

JF - Gene

IS - 2

ER -

Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

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Dissecting Gene expression Networks

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