Different pathways act downstream of the peptide receptor CRA2 to regulate lateral root and nodule development

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DOI

  • Nadiatul A Mohd-Radzman
  • ,
  • Carole Laffont
  • ,
  • Ariel Ivanovici
  • ,
  • Neha Patel
  • ,
  • Dugald Elgin Reid
  • Jens Stougaard
  • Florian Frugier
  • ,
  • Nijat Imin
  • ,
  • Michael Anthony Djordjevic

C-TERMINALLY ENCODED PEPTIDEs (CEPs) control root system architecture non-cell-autonomously. In Medicago truncatula, MtCEP1 affects root development by increasing nodule formation and inhibiting lateral root emergence by unknown pathways. Here, we show that the MtCEP1 peptide-dependent increase in nodulation requires the symbiotic (SYM) signalling pathway and MtEIN2 (ETHYLENE INSENSITIVE2)/SKL (SICKLE), but acts independently of SUNN (SUPER NUMERIC NODULES). MtCEP1-dependent inhibition of lateral root development acts through an MtEIN2-independent mechanism. MtCEP1 increases nodulation by promoting rhizobial infections, the developmental competency of roots for nodulation, the formation of "fused" nodules, and an increase in frequency of nodule development that initiates at proto-phloem poles. These phenotypes are similar to those of the ein2/skl mutant and support that MtCEP1 modulates EIN2-dependent symbiotic responses. Accordingly, MtCEP1 counteracts the reduction in nodulation induced by increasing ethylene precursor concentrations, and an ethylene synthesis inhibitor treatment antagonises MtCEP1 root phenotypes. MtCEP1 also inhibits the development of EIN2-dependent pseudonodule formation. Finally, mutants affecting the MtCRA2 (COMPACT ROOT ARCHITECTURE 2) receptor, which is closely related to the Arabidopsis CEP Receptor 1, are unresponsive to MtCEP1 effects on lateral root and nodule formation, suggesting that CRA2 is a CEP peptide receptor mediating both organogenesis programs. In addition, an ethylene inhibitor treatment counteracts the cra2 nodulation phenotype. These results indicate that MtCEP1 and its likely receptor, CRA2, mediate nodulation and lateral root development through different pathways.

Original languageEnglish
JournalPlant Physiology
Volume171
Issue4
Pages (from-to)2536-2548
Number of pages13
ISSN0032-0889
DOIs
Publication statusPublished - 24 Jun 2016

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