Development of single-cell transcriptomic atlas of human plasmacytoid dendritic cells from people with HIV-1

Lamin B Cham*, Lin Lin*, Martin Tolstrup, Ole S Søgaard*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Many immunological treatment strategies for reducing the HIV-1 reservoir and enhancing adaptive immunity aim at activating the human plasmacytoid dendritic cells (pDCs). Here, we present a protocol for pDC enrichment, single-cell analysis, and development of a pDC transcriptomic database from healthy individuals and people with HIV-1 before and after Toll-like receptor 9 agonist treatment. For complete details on the use and execution of this protocol, please refer to Cham et al.1

Original languageEnglish
Article number102777
JournalSTAR Protocols
Volume5
Issue1
Pages (from-to)102777
DOIs
Publication statusPublished - 15 Mar 2024

Keywords

  • Bioinformatics
  • Immunology
  • Adaptive Immunity
  • Dendritic Cells
  • Interferon-alpha
  • HIV-1/genetics
  • Humans
  • Gene Expression Profiling

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